Crops ›› 2016, Vol. 32 ›› Issue (3): 58-62.doi: 10.16035/j.issn.1001-7283.2016.03.011

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Transformation of GmPHR1 and GmPAP4 Related to High Phosphorus Efficiency and Elite Germplasm Enhancement in Soybean (Glycine max)

Geng Zhao,Kong Youbin,Zhao Lili,Liu Cui,Du Hui,Li Xihuan,Zhang Caiying   

  1. College of Agronomy,Agriculture University of Hebei/North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Baoding 071001,Hebei,China
  • Received:2016-03-03 Revised:2016-04-01 Online:2016-06-15 Published:2018-08-26
  • Contact: Xihuan Li

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Abstract:

Phosphorus is essential for soybean growth and development, and lack of phosphorus in soil has become an important limiting factor for high yield and quality in soybean. Most previous studies focus on transformation of single-gene related to phosphorus efficiency, while the transformation of double-genes into a plant was rarely reported in soybean. In this study, we used transgenic soybean plants with GmPAP4 or GmPHR1 to achieve new transgenic germplasms with double gene (GmPAP4 and GmPHR1) in a plant via Agrobacterium-mediated transformation and conventional hybridization techniques. It was found that both the methods could achieve co-transformation double-genes plants in variety JD12, and the positive plants were verified further by PCR and DNA sequencing methods. Thus, the new transgenic germplasms with GmPHR1 and GmPAP4 named “JD12-PHR1-PAP4” was obtained.

Key words: Soybean, Low phosphate stress, GmPHR1, GmPAP4, Double gene co-transformation

Fig.1

PCR identification of JD12-PHR1"

Fig.2

Southern blotting results of JD12-PHR1"

Fig.3

Relative expression of GmPHR1in transgenic JD12-PHR1"

Fig.4

PCR identification results of JD12-PHR1-PAP4 with double genes"

Fig.5

DNA sequencing results of JD12-PHR1-PAP4 with double genes"

Fig.6

PCR identification of transgenic soybean plants with GmPHR1 and GmPAP4"

[1] Lu S . Study on the adsorption and desorption of phosphorus in soils and phosphorus requirement. Plant Nutrition & Fertilizing Science, 19951(3-4):29-35.
[2] Zhang Y L . Analysis of phosphorus nutrition efficiency of soybean genotypes with different phosphorus efficiency at blooming stage under low phosphorus stress. Agricultural Science & Technology, 2012,13(12):2544-2548.
[3] Nilsson L, Muller M, Nielsen T H . Increased expression of the MYB-related transcription factor,PHR1,leads to enhanced phosphate uptake in Arabidopsis thaliana. Plant Cell & Environment, 2007,30(12):1499-1512.
[4] 邢国芳, 郭平毅 .植物响应低磷胁迫的功能基因组研究进展.生物技术通报,2013(7):1-6.
[5] 李宝健, 朱华晨 . 论应用多基因转化策略综合改良生物体遗传性研究方向的前景Ⅰ.多基因转化的基因来源与技术平台. 中山大学学报(自然科学版), 2004,43(6):11-16.
[6] 李宝健, 朱华晨 . 论应用多基因转化策略综合改良生物体遗传性研究方向的前景Ⅱ.多基因转化策略中的规律、前景和问题. 中山大学学报(自然科学版), 2005,44(4):79-83.
[7] 邓小梅, 赵先海, 袁金英 , 等.植物多基因转化方法研究进展.林业科技开发,2014(2):7-12.
doi: 10.13360/j.issn.1000-8101.2014.02.002
[8] Naqvi S, Farré G, Sanahuja G , et al. When more is better:multigene engineering in plants. Trends in Plant Science, 2010,15(1):48-56.
doi: 10.1016/j.tplants.2009.09.010 pmid: 19853493
[9] 李环, 丁昌俊, 苏晓华 , 等. 涝渍胁迫对转多基因库安托杨生长及生理性状的影响. 林业科学研究, 2010,23(1):44-52.
[10] 魏睿, 麻冬梅, 许兴 .转多基因草坪草的耐盐性鉴定.北方园艺,2013(7):59-62.
[11] 常雪, 鲁新, 王伟 , 等.转cry1Ab/cry1I玉米对亚洲玉米螟的抗性评价// 2014年中国植物保护学会学术年会论文集, 2014.
[12] 吴冰, 李喜焕, 刘翠 , 等. 大豆MYB转录因子GmPHR1转化及功能研究. 大豆科学, 2013,32(3):304-305.
[13] Li X H, Wang Y J, Wu B , et al. GmPHR1,a novel homolog of the AtPHR1 transcription factor,plays a role in plant tolerance to phosphate starvation. Journal of Integrative Agriculture, 2014,13(12):2584-2593.
doi: 10.1016/S2095-3119(14)60775-9
[14] Kong Y B, Li X H, Ma J , et al. GmPAP4,a novel purple acid phosphatase gene isolated from soybean (Glycine max),enhanced extracellular phytate utilization in Arabidopsis thaliana. Plant Cell Reports, 2014,33(4):655-667.
doi: 10.1007/s00299-014-1588-5
[15] 赵莉莉, 李喜焕, 李文龙 , 等. MYB转录因子AtPHR1转化大豆研究. 大豆科学, 2013,32(2):154-159.
[16] 王伟, 王罡, 季静 , 等.大豆子叶节植株再生体系的优化及转EPSPS基因的研究.作物杂志,2012(3):23-27.
[17] 李海燕, 刘淼, 武小霞 , 等.大豆转化过程中的褐化现象研究.作物杂志,2010(1):33-36.
[18] 别晓敏, 佘茂云, 杜丽璞 , 等. 植物多基因转化研究进展. 中国农业科技导报, 2010,12(6):18-23.
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