Crops ›› 2017, Vol. 33 ›› Issue (1): 140-143.doi: 10.16035/j.issn.1001-7283.2017.01.025

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Effects of Acid Stress on Microtuberization and Some Glycometabolism of Potato in Vitro Plantlets

Teng Yue1,Gao Yuliang2,Zhang Yan1,Jin Meiyu1,Li Kuihua1   

  1. 1Agricultural College of Yanbian University,Yanji 133002,Jilin,China
    2Yanbian Agricultural Sciences Academy,Longjing 133400,Jilin,China
  • Received:2016-11-02 Revised:2016-12-19 Online:2017-02-15 Published:2018-08-26

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Abstract:

The transfer of in-vitro potato plantlets in pH 3 solution to the MS medium containing 30g/L sucrose which was against formation potato tuber was conducted to induce the microtuber and evaluate the contents of sucrose and starch potato plantlets as well as the changes in sucrose synthetase (SS), soluble starch synthetase (SSS) and ADP-glucose pyrophosphorylase (AGPase) activity during the induction. Results indicated that a short induction time and the highest number of potato microtubers occurred from potato plantlets under stress, however untreated plantlets failed to induce tubers.For microtuber formation after pH 3 stress for 1d, microtuberization starts at the 13th day in MS medium, and the number of microtubers for a single plantlet was 2.2 and the mean weight of microtuber was 187.8mg. No significant change was found in sucrose content, but SS, SSS, and AGPase activities increased significantly in leaves during acid stress treatment. SS activity decreased and increased alternately,SSS activity decreased and then increased at 0-5d, but later tended to stabilize. AGPase activity in leaves of induced microtuber tended to increase and then decrease but later increased in leaves of plantlet with acid stress during inducing microtuber.

Key words: Potato, Microtuber, Acid stress, Glycometabolism

Table 1

Influences of acid stress (pH=3) time on induction of potato microtuber"

胁迫天数(d)
Days of stress		 结薯始期(d)
Days of microtuber initiation		 单株结薯数
No.of microtuber per plantlet		 单薯重(mg)
Weight of microtuber		 单株出芽量
No.of shoot per plantlet
1 13 2.2a 187.8a 2.6c
2 15 1.3b 131.3b 3.8b
3 15 1.1c 124.6c 3.6b
0 (CK) - - - 5.4a

Fig.1

Changes in plant sucrose content and SS activity for potato plantlets Different capital and small letters indicate significant differences at the 0.05 level among induction days under the same treatment,the same below"

Fig.2

Changes of starch content,SSS activity and AGPase activity for potato plantlets"

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