Crops ›› 2018, Vol. 34 ›› Issue (2): 73-79.doi: 10.16035/j.issn.1001-7283.2018.02.013

Previous Articles     Next Articles

Effects of Different Ratio of N to K on Carbon-Nitrogen Metabolism and Chemical Constituents of Dark Sun-Cured Tobacco

Zhao Bingfei1,Sun Shuang1,Hou Zhenwu2,Fu Yunpeng1,Fu Quanshan2,Yang Yongfeng3,Wang Jing1,Huang Yongcheng4   

  1. 1 College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
    2 Jilin Jiaohe Tobacco Co.Ltd., Jiaohe 132500, Jilin, China
    3 Henan Tobacco Co. Ltd., Zhengzhou 450000, Henan, China
    4 Changchun Branch of Jilin Tobacco Company, Changchun 130000, Jilin, China
  • Received:2017-11-13 Revised:2017-12-15 Online:2018-04-20 Published:2018-08-27

RichHTML

3

PDF (PC)

142

Abstract:

Effects of different ratio of nitrogen to potassium on chromoplast pigment, carbon-nitrogen metabolism and chemical constituents of dark sun-cured tobacco were studied in Jiaohe. The results showed that with the nitrogen increased, the contents of chlorophyll and activity of nitrate reductase in dark sun-cured tobacco leaves increased. Under nitrogen level of 90kg/hm 2 and the ratio of N to K was 1:2, the content of chlorophyll and carotenoids was at a high level at later stage, which was harmful to the normal metabolism of carbon-nitrogen and tobacco maturing. However, nitrogen level of 75kg/hm 2 and the ratio of N to K level of 1:3 were good for carbon-nitrogen metabolism. In the term of chemical constituents of cured tobacco. with the increase of nitrogen level, the content of nitrogen compounds increased and the carbohydrate content decreased. With the increase of potassium level, the potassium content in cured tobacco increased obviously. Therefore, under sufficient nitrogen supply, it was beneficial to tobacco quality with increasing the proportion of potassium in tobacco planting soil.

Key words: Dark sun-cured tobacco, N, K ratio, Carbon-nitrogen metabolism, Chemical constituents

Table 1

The experiment treatment combination"

处理
Treatment		 N
(kg/hm2)		 P2O5
(kg/hm2)		 K2O
(kg/hm2)		 N∶K2O
T1 (低氮低钾) N1(60) P(90) K1(120) 1∶2
T2 (低氮高钾) N1(60) P(90) K2(180) 1∶3
T3 (中氮低钾) N2(75) P(90) K1(150) 1∶2
T4 (中氮高钾) N2(75) P(90) K2(225) 1∶3
T5 (高氮低钾) N3(90) P(90) K1(180) 1∶2
T6 (高氮高钾) N3(90) P(90) K2(270) 1∶3

Fig.1

The effects of diffierent ratio of N, K on the activity of NR"

Table 2

The analysis of variance of NR activity on 30 days of tabacco leaf"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of squares		 均方
Mean squares		 F值
F value		 F0.05
主区Whole plot 区组Block 2 0.05 0.03 0.62 6.94
氮用量Nitrogen rate 2 111.46 55.73 1 293.62* 6.94
误差Error 4 0.17 0.04
总变异Total variation 8 111.68
副区Split plot 氮钾配比N, K ratio 1 0.41 0.41 8.54* 5.99
氮用量×氮钾配比Nitrogen rate×N, K ratio 2 0.38 0.19 4.03 5.14
误差Error 6 0.29 0.05
总变异Total variation 17 112.76

Fig.2

The effects of diffierent ratio of N, K on the activity of INV"

Table 3

The analysis of variance of INV activity on 20 days of tabacco leaf"

变异来源
Source of variation		 自由度
Degree of freedom		 平方和
Sum of squares		 均方
Mean squares		 F值
F value		 F0.05
主区Whole plot 区组Block 2 0.15 0.07 5.68 6.94
氮用量Nitrogen rate 2 19.47 9.73 23 358.24* 6.94
误差Error 4 0.00 0
总变异Total variation 8 19.62
副区Split plot 氮钾配比N, K ratio 1 0.59 0.59 1 021.88* 5.99
氮用量×氮钾配比Nitrogen rate×N, K ratio 2 0.30 0.15 262.65* 5.14
误差Error 6 0.00 0
总变异Total variation 17 20.51

Fig.3

The effects of diffierent ratio of N, K on the content of chlorophyll"

Fig.4

The effects of diffierent ratio of N, K on the content of carotenoids"

Table 4

The effects of diffierent ratio of N, K on chemical components in middle leaves of dark sun-cured tobacco"

处理
Treatment		 总氮(%)
Total nitrogen		 烟碱(%)
Nicotine		 总糖(%)
Total sugar		 还原糖(%)
Reducing sugar		 氯(%)
Chlorine		 钾(%)
Potassium		 糖碱比
Reducing sugar/Nicotine		 氮碱比
Total nitrogen/Nicotine
T1 2.48cC 2.85cdC 9.56bB 9.07bB 0.25eE 1.68cBC 3.18 0.87
T2 2.24dD 2.84cdC 10.49aA 10.09aA 0.37dD 1.85bB 3.55 0.79
T3 2.70bB 2.93cC 8.04cC 7.30dD 0.55bB 1.81bBC 2.49 0.92
T4 2.61bBC 2.82dC 8.24cC 8.03cC 0.38dD 2.03aA 2.85 0.93
T5 3.13aA 3.48aA 6.86dD 6.28fF 0.48cC 1.45dD 1.80 0.90
T6 3.09aA 3.28bB 6.95dD 6.78eE 0.67aA 1.67cC 2.07 0.94

Table 5

The effects of diffierent ratio of N, K on the contents of aroma consituents of dark sun-cured tobacco μg/g"

类别Categories 化合物名称Compound names T1 T2 T3 T4 T5 T6
棕色化反应产物 3,4-二甲基-2,5-呋喃3,4-dimethyl-2,5-furan 5.49 8.82 5.36 9.77 6.45 7.18
Browning reaction products 糠醛Furfural 29.54 69.53 45.49 70.59 32.69 77.05
糠醇Furfural alcohol 4.61 8.43 5.28 10.22 6.65 7.48
2-乙酰呋喃2-acetyl furan 0.56 1.07 0.81 1.02 0.63 1.11
2-乙酰吡咯2-pyrrole acetyl groups 0.28 0.41 0.40 0.49 0.19 0.27
5-甲基糠醛5-methyl-2-furfural 0.47 0.85 0.92 1.30 1.13 1.61
小计Total 40.95 89.11 58.26 93.39 47.74 94.70
类胡萝卜素降解产物 6-甲基-5-庚烯-2-酮6-methyl-5-heptene-2-dioe 4.70 8.64 5.16 7.27 6.06 6.78
Carotenoid degradation products 芳樟醇Linalool 7.75 11.10 8.99 9.28 8.88 8.97
6-甲基-5-庚烯-2-醇6-methyl-5-heptene-2-alocohol 1.44 2.61 2.10 2.11 1.90 1.75
氧化异佛尔酮Isophorone oxide 0.58 0.92 0.59 0.73 0.72 0.72
2,6-壬二烯醛2,6- nonyladienal 0.93 1.03 0.66 1.72 1.07 1.28
异佛尔酮Isophorone 1.24 2.12 1.47 2.02 1.62 1.52
β-大马酮β-damascenone 11.63 18.28 12.36 19.77 16.78 20.31
β-二氢大马酮β-dihydro damascenone 6.43 10.52 7.48 11.44 6.72 12.71
香叶基丙酮Geranylacetone 9.01 13.44 10.85 12.80 10.99 11.28
3-羟基-β-二氢大马酮3-hydroxy-β-damascenone 18.30 24.65 19.32 22.10 19.95 20.71
巨豆三烯酮1 Megastigmatrienone1 0.97 1.37 1.36 1.26 1.11 1.37
巨豆三烯酮2 Megastigmatrienone2 3.11 4.24 3.00 4.66 3.87 4.47
巨豆三烯酮3 Megastigmatrienone3 3.75 4.81 6.64 4.56 2.73 6.40
巨豆三烯酮4 Megastigmatrienone4 3.08 5.50 4.66 5.21 4.93 6.75
螺岩兰草酮Solavetivone 71.15 64.53 67.05 95.46 70.57 98.83
法尼基丙酮Famesy acetore 22.81 39.95 31.10 37.29 31.03 37.31
二氢猕猴桃内酯Dihydro actinidiolide 9.10 14.76 9.90 10.27 11.14 10.77
藏花醛Safranal 1.09 1.47 1.18 1.20 1.19 0.91
小计Total 169.32 218.84 184.88 239.87 192.38 243.87
苯丙氨酸降解产物 苯甲醛Benzaldehyde 2.26 0.82 2.03 1.36 2.01 1.22
Phenylalanine degradation products 苯乙醛Phenylacetaldehyde 3.43 5.15 7.80 5.90 5.01 6.28
苯甲醇Benzyl alcohol 14.78 25.36 15.02 28.31 13.52 19.87
苯乙醇Phenylethyl alcohol 20.96 32.30 21.91 30.54 23.50 31.86
小计Total 41.43 63.63 46.76 66.11 44.04 59.23
西柏烷类降解产物
Alkanes degradation products		 茄酮Solanone 30.31 39.22 34.23 56.63 39.67 44.47
叶绿素降解产物
Degradation products of chlorophyll		 新植二烯Neophytadiene 242.11 320.42 295.88 367.80 341.51 442.33
除新植二烯外中性香气物质总量Total neutral aroma components except for neophytadiene 289.76 421.90 333.12 465.28 332.71 451.24
中性香气成分总量 Total neutral aroma components 531.87 742.32 629.00 833.08 674.22 893.57
[1] 马兴华, 石屹, 张忠锋 , 等. 施氮量与基追比例对烟叶品质及氮肥利用率的影响. 中国烟草科学, 2015(4):34-39.
doi: 10.13496/j.issn.1007-5119.2015.04.006
[2] 陈莹, 谭建, 杨建志 , 等. 不同施氮水平对特色烟叶品质和产量的影响. 贵州农业科学, 2012(4):88-90.
doi: 10.3969/j.issn.1001-3601.2012.04.025
[3] 高琴, 刘国顺, 李姣 , 等. 不同氮肥水平对烤烟质体色素和碳氮代谢及品质的影响. 河南农业大学学报, 2013(2):138-142.
[4] 王衬, 毛黎娟, 惠非琼 , 等. 不同氮素水平下内生真菌印度梨形孢定殖对烟草生长发育及物质代谢的影响. 烟草科技, 2014(5):76-81,98.
doi: 10.3969/j.issn.1002-0861.2014.05.017
[5] 沈方科, 李婷, 王蕾 , 等. 钾素营养对烟株氮代谢及烟叶品质形成的影响. 中国农学通报, 2010(9):214-219.
[6] 闫慧峰, 石屹, 李乃会 , 等. 烟草钾素营养研究进展. 中国农业科技导报, 2013(1):123-129.
doi: 10.3969/j.issn.1008-0864.2013.01.20
[7] 刘文祥, 颜合洪, 周益 , 等. 烟草钾素营养与提高烤烟烟叶含钾量的研究进展. 作物研究, 2007(s1):736-740.
[8] 王念磊, 符云鹏, 贾保顺 , 等. 氮素对不同品种烤烟质体色素及其降解产物含量的影响. 山东农业科学, 2015(7):68-73.
doi: 10.14083/j.issn.1001-4942.2015.07.015
[9] 康雪莉 . 吉林晒烟生长发育及碳氮代谢与品质关系研究. 郑州:河南农业大学, 2014.
[10] 邹琦 . 植物生理生化实验指导.北京: 中国农业出版社, 1995.
[11] 刘雪松, 刘贞琦, 赵振刚 , 等. 烟草生育期光合特性的变化. 烟草学刊, 1991(2):23-29.
[12] 方明, 符云鹏, 刘国顺 , 等. 磷钾配施对晒红烟碳氮代谢和光合效率的影响. 中国烟草科学, 2007(2):27-30.
[13] 史宏志, 韩锦峰, 刘国顺 , 等. 烤烟碳氮代谢与烟叶香吃味关系的研究. 中国烟草学报, 1998(2):56-63.
[14] 常思敏, 马新明, 张贵龙 , 等. 砷对烤烟碳氮代谢及其产量和品质的影响. 植物生态学报, 2006(4):682-688.
doi: 10.17521/cjpe.2006.0089
[15] 郭濛濛, 邱宝平, 符云鹏 , 等. 氮磷钾配施比例对晒红烟碳氮代谢关键酶活性及化学成分的影响. 江苏农业科学, 2015,43(5):95-98.
[16] 李洪臣, 杨志晓, 武云杰 , 等. 氮肥用量和施用方式对烟草中部叶碳氮代谢的影响. 江苏农业科学, 2013,41(3):65-68.
doi: 10.3969/j.issn.1002-1302.2013.03.024
[17] 杨志哓, 史跃伟, 林世峰 , 等. 烤烟碳氮代谢关键酶活性动态及其与类胡萝卜素关系研究. 中国烟草科学, 2014,35(2):59-63.
[18] 景延秋, 宫长荣, 张月华 , 等. 烟草香味物质分析研究进展. 中国烟草科学, 2005,26(2):44-48.
doi: 10.3969/j.issn.1007-5119.2005.02.014
[19] 史宏志, 韩锦峰 . 烤烟碳氮代谢几个问题的探讨. 烟草科技, 1998(2):34-36.
[1] Wu Hao, Li Yanmin, Xie Chuanxiao. Research Advances on Physiological Basis and Gene#br# Discovery for Thermal Tolerance in Crops [J]. Crops, 2018, 34(5): 1-9.
[2] Wu Ruixiang, Yang Jianchun, Wang Liqin, Guo Xiujuan. Evaluation of the Adaptability of Flax Drought#br# Resistance Based on Multiple Statistics Analysis [J]. Crops, 2018, 34(5): 10-16.
[3] Ji Shengdong, Li Peng, Li Jiangwei, Song Liumin, . Analysis of Peroxidase Zymogram and Genetic#br# Effects between Rice Lines and Their#br# Parents During Grain Filling [J]. Crops, 2018, 34(5): 17-20.
[4] Ma Mengli, Zheng Yun, Zhou Xiaomei, . Genetic Diversity Analysis of Red Rice from#br# Hani’s Terraced Fields in Yunnan Province [J]. Crops, 2018, 34(5): 21-26.
[5] Chen Guangzhou, Wang Guangfu, Qu Jianzhou, Si Leiyong, . Study on Grain Dehydration Rate and#br# Correlation Analysis of Major Related#br# Characters in Different Maize Inbred Lines [J]. Crops, 2018, 34(5): 33-39.
[6] Wang Hanxia, Shan Fuhua, Tian Liping, Ma Qiaoyun, . Analysis of Stability and Adaptability of Winter#br# Wheat Varieties in the Regional Trials of#br# the Northern Wheat Region of China [J]. Crops, 2018, 34(5): 40-44.
[7] Wang Lei, Zhang Xiangping, Li Runxi, Niu Xiaoxia, . Multivariate Analysis and Evaluation on Agronomic#br# Traits and Grain Amylopectin Content of Barley [J]. Crops, 2018, 34(5): 71-76.
[8] Zhang Yizhong, Zhou Fuping, Zhang Xiaojuan, . Identification and Cluster Analysis of Photosynthetic#br# Characters and WUE in Sorghum Germplasm [J]. Crops, 2018, 34(5): 45-53.
[9] Ma Jianhui, Zhang Wenli, Gao Xiaolong, Zhang Daijing, . Identification and Expression Analysis of the Whole#br# Glutathione S-Transferase Genome Family in#br# Aegilops tauschii under Abiotic Stress [J]. Crops, 2018, 34(5): 54-62.
[10] Su Guihua, Li Chunlei, Su Yichen. Evaluation of 22 Main Popularized Varieties#br# by Variety Regional Trails in Jilin Province [J]. Crops, 2018, 34(5): 63-70.
[11] Wang Fengfeng, Wang Ping, Chen Li, . Analysis of Morphological Diversity Based on#br# Botanical Traits in Seed-Used Pumpkin Germplasms [J]. Crops, 2018, 34(5): 77-84.
[12] Tang Liyuan, Li Xinghe, Zhang Sujun, Wang Haitao, . QTL Mapping for Photosynthesis#br# Related Traits in Upland Cotton [J]. Crops, 2018, 34(5): 85-90.
[13] Zhang Xiangyu, Li Hai, Liang Haiyan, . Effects of Different Row Spacing and Planting Density#br# on the Growth Characteristics and Yield of Millet [J]. Crops, 2018, 34(5): 91-96.
[14] Wu Ronghua, Zhuang Kezhang, Liu Peng, Zhang Chunyan. Response of Summer Maize Yield to#br# Meteorological Factors in Lunan Region [J]. Crops, 2018, 34(5): 104-109.
[15] Su Feifei, Zhang Jinghua, Li Yong, Liu Shangwu, Liu Zhenyu, Wang Shaopeng, Wan Shuming, Chen Xi, Gao Yunfei, Hu Linshuang, Lü Dianqiu. Effects of Different Irrigation Methods on#br# Physiological Characteristics and Water#br# Use Efficiency of Potato [J]. Crops, 2018, 34(5): 97-103.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] Guangcai Zhao,Xuhong Chang,Demei Wang,Zhiqiang Tao,Yanjie Wang,Yushuang Yang,Yingjie Zhu. General Situation and Development of Wheat Production[J]. Crops, 2018, 34(4): 1 -7 .
[2] Baoquan Quan,Dongmei Bai,Yuexia Tian,Yunyun Xue. Effects of Different Leaf-Peg Ratio on Photosynthesis and Yield of Peanut[J]. Crops, 2018, 34(4): 102 -105 .
[3] Xuefang Huang,Mingjing Huang,Huatao Liu,Cong Zhao,Juanling Wang. Effects of Annual Precipitation and Population Density on Tiller-Earing and Yield of Zhangzagu 5 under Film Mulching and Hole Sowing[J]. Crops, 2018, 34(4): 106 -113 .
[4] Wenhui Huang, Hui Wang, Desheng Mei. Research Progress on Lodging Resistance of Crops[J]. Crops, 2018, 34(4): 13 -19 .
[5] Yun Zhao,Cailong Xu,Xu Yang,Suzhen Li,Jing Zhou,Jicun Li,Tianfu Han,Cunxiang Wu. Effects of Sowing Methods on Seedling Stand and Production Profit of Summer Soybean under Wheat-Soybean System[J]. Crops, 2018, 34(4): 114 -120 .
[6] Mei Lu,Min Sun,Aixia Ren,Miaomiao Lei,Lingzhu Xue,Zhiqiang Gao. Effects of Spraying Foliar Fertilizers on Dryland Wheat Growth and the Correlation with Yield Formation[J]. Crops, 2018, 34(4): 121 -125 .
[7] Xiaofei Wang,Haijun Xu,Mengqiao Guo,Yu Xiao,Xinyu Cheng,Shuxia Liu,Xiangjun Guan,Yaokun Wu,Weihua Zhao,Guojiang Wei. Effects of Sowing Date, Density and Fertilizer Utilization Rate on the Yield of Oilseed Perilla frutescens in Cold Area[J]. Crops, 2018, 34(4): 126 -130 .
[8] Pengjin Zhu,Xinhua Pang,Chun Liang,Qinliang Tan,Lin Yan,Quanguang Zhou,Kewei Ou. Effects of Cold Stress on Reactive Oxygen Metabolism and Antioxidant Enzyme Activities of Sugarcane Seedlings[J]. Crops, 2018, 34(4): 131 -137 .
[9] Jie Gao,Qingfeng Li,Qiu Peng,Xiaoyan Jiao,Jinsong Wang. Effects of Different Nutrient Combinations on Plant Production and Nitrogen, Phosphorus and Potassium Utilization Characteristics in Waxy Sorghum[J]. Crops, 2018, 34(4): 138 -142 .
[10] Na Shang,Zhongxu Yang,Qiuzhi Li,Huihui Yin,Shihong Wang,Haitao Li,Tong Li,Han Zhang. Response of Cotton with Vegetative Branches to Plant Density in the Western of Shandong Province[J]. Crops, 2018, 34(4): 143 -148 .