Crops ›› 2019, Vol. 35 ›› Issue (1): 44-49.doi: 10.16035/j.issn.1001-7283.2019.01.007

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Genome-Wide Association Analysis of Wheat at Heading and Flowering Stages

Zheng Zhang1,Yinquan Niu1,Dong Zhang1,Chengmei Hu1,Yichuan Yuan1,Huiyan Wang1,Shuguang Wang1,Yaping Cao2,Daizhen Sun1   

  1. 1 College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China
    2 Wheat Research Institute, Shanxi Academy of Agricultural Sciences, Linfen 041000, Shanxi, China
  • Received:2018-08-08 Revised:2019-01-02 Online:2019-02-15 Published:2019-02-01
  • Contact: Daizhen Sun

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Abstract:

In order to reveal the genetic loci controlling the heading and flowering date of wheat, 106 pairs of SSR markers, evenly distributed on 21 chromosomes, and a natural population consisted of 205 accessions, were used to perform genome-wide association analysis. The results showed that 879 allelic variations were detected in 106 pairs of SSR markers. The main allelic variation frequency ranged from 0.175 to 0.986 with an average of 0.548. The polymorphism information index ranged from 0.028-0.900 with an average of 0.557. A mixed linear model was used to detect seven loci associated with significant heading and flowering date (P<0.01), one of which were extremely significant (P<0.001). In addition, Xgwm182(5D) was significantly associated with heading date under irrigation conditions. Xbarc42(3D) was significantly associated with heading and flowering date under rainfed conditions, respectively. These two sites were consistent with previous studies. Xgwm102(2D) was significantly associated with flowering date under irrigation conditions, while Xgwm124(1B) was significantly associated with heading date under rainfed conditions. Xgwm130(7A) was associated with heading date under rainfed and irrigation conditions. The above results provide information for molecular marker-assisted selection breeding of heading and flowering date in wheat.

Key words: Wheat, Heading date, Flowering date, Association analysis, Marker-assisted selection breeding

Table 1

Descriptive statistics for different phenotypic data"

年份
Year		 环境
Environment		 性状
Trait		 平均值(d)
Average		 标准差
Standard deviation		 变幅(d)
Variable amplitude		 变异系数(%)
Coefficient of variation
2017 雨养 抽穗期 216.60 2.17 213~223 1.00
开花期 222.36 1.78 218~227 0.80
灌溉 抽穗期 217.28 2.36 214~224 1.09
开花期 223.72 1.83 217~229 0.82
2018 雨养 抽穗期 239.23 2.31 235~247 0.97
开花期 244.21 1.60 241~250 0.66
灌溉 抽穗期 241.91 2.07 237~249 0.86
开花期 247.34 1.31 244~251 0.53

Fig.1

Analysis results of population structure of 205 wheat varieties based on 106 SSR markers b, genetic structure analyzed by Structure V2.3.2 software"

Table 2

Association analysis of heading and flowering date in wheat"

生育期
Growing stage		 标记
Marker		 染色体
Chromosome		 年份
Year		 环境
Environment		 P值
P value		 表型解释率R2 (%)
Phenotypic interpretation rate R2		 等位变异(bp)
Allelic variation		 效应(d)
Effect
抽穗期Heading gwm182 5D 2017 灌溉 0.00721 7.62 160 -1.29
gwm130 7A 2018 雨养 0.00702 10.90 132 1.62
gwm130 7A 2018 灌溉 0.00305 12.18 132 2.04
barc42 3D 2018 雨养 0.00537 5.26 112 -1.85
gwm124 1B 2018 雨养 0.00312 8.10 190 -0.95
开花期Flowering gwm102 2D 2017 灌溉 0.00044 14.31 134 2.96
gwm218 3A 2017 灌溉 0.00636 11.32 136 -1.68
barc42 3D 2018 雨养 0.00537 5.26 112 -1.26
[1] 宋彦霞, 景蕊莲, 霍纳新 , 等 . 普通小麦 (T. aestivum L.)不同作图群体抽穗期QTL分析. 中国农业科学, 2017(11):2186-2193.
doi: 10.3321/j.issn:0578-1752.2006.11.004
[2] 宋彦霞 . 小麦抽穗期及其它农艺性状的QTL分析. 雅安:四川农业大学, 2005.
[3] 闫雪, 史雨刚, 李晓宇 , 等. 小麦开花期QTL分析. 山西农业科学, 2015,43(8):919-921,950.
doi: 10.3969/j.issn.1002-2481.2015.08.01
[4] 谷俊涛, 刘桂茹, 栗雨勤 , 等. 不同抗旱类型小麦品种开花期光合速率与抗旱性的比较研究. 河北农业大学学报, 2017(3):1-4.
doi: 10.3969/j.issn.1000-1573.2001.03.001
[5] 何龙海 . 水稻抽穗期基因位点的连锁与关联分析. 南昌:江西农业大学, 2014.
[6] Mackay I, Powell W . Methods for linkage disequllibrium mapping in crops. Trends in Plant Science, 2007,12(2):57-63.
doi: 10.1016/j.tplants.2006.12.001 pmid: 17224302
[7] Qaseem M F, Qureshi R, Muqaddasi Q H , et al. Genome-wide association mapping in bread wheat subjected to independent and combined high temperature and drought stress. PLoS ONE, 2018,13(6):e0199121.
doi: 10.1371/journal.pone.0199121 pmid: 29949622
[8] 刘坤, 张雪海, 孙高阳 , 等. 玉米株型相关性状的全基因组关联分析. 中国农业科学, 2018,51(5):821-834.
[9] 李婉琳, 宋洁, 郭文 , 等. 马铃薯主要农艺性状的SSR标记关联分析. 分子植物育种, 2016,14(11):3102-3112.
[10] 赵波, 叶剑, 金文林 , 等. 不同类型小豆种质SSR标记遗传多样性及性状关联分析. 中国农业科学, 2011,44(4):673-682.
doi: 10.3864/j.issn.0578-1752.2011.04.003
[11] 赖勇, 吕仲昱, 贾建磊 , 等. 外引大麦农艺性状SSR关联位点及等位变异表型效应分析. 麦类作物学报, 2018,38(4):424-429.
doi: 10.7606/j.issn.1009-1041.2018.04.07
[12] 邹德堂, 赵广欣, 孙健 . 水稻淀粉合成候选基因OsAGPL3与淀粉相关性状的关联分析. 东北农业大学学报, 2017,48(9):1-10.
doi: 10.3969/j.issn.1005-9369.2017.09.001
[13] 郭杰 . 小麦育成品种穗顶部、基部结实性及其它产量相关性状的遗传解析. 南京:南京农业大学, 2015.
[14] Somers D J, Isaac P, Edwards K . A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 2017(10):1105-1114.
doi: 10.1007/s00122-004-1740-7 pmid: 15490101
[15] Liu K, Muse S V . PowerMarker:an integrated analysis environment for genetic marker analysis. Bioinformatics, 2005,21(9):2128-2129.
doi: 10.1093/bioinformatics/bti282 pmid: 15705655
[16] Pritchard J K, Stephens M, Rosenberg N A , et al. Association mapping in structured populations. American Journal of Human Genetics, 2000,67(1):170-180.
doi: 10.1086/302959 pmid: 10827107
[17] Evanno G, Regnaut S, Goudet J . Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study. Molecular Ecology, 2005,14(8):2611-2620.
doi: 10.1111/j.1365-294X.2005.02553.x pmid: 15969739
[18] Hardy O J, Vekemans X . SPAGeDi:a versatile computer program to analyze spatial genetic structure at the individual or population levels. Molecular Ecology, 2002,2(4):618-620.
doi: 10.1046/j.1471-8286.2002.00305.x
[19] Bernardo R . Best linear unbiased prediction of maize single-cross performance. Crop Science, 1996,36(1):50-56.
doi: 10.2135/cropsci1996.0011183X003600010009x
[20] 胡建帮 . 262份小麦自然群体抽穗期的关联分析. 合肥:安徽农业大学, 2015.
[21] Zanke C, Ling J, Plieske J , et al. Genetic architecture of main effect QTL for heading date in European winter wheat. Frontiers in Plant Science, 2014,5:217.
doi: 10.3389/fpls.2014.00217 pmid: 4033046
[22] 张嘉楠 . 小麦资源材料抗旱相关性状关联分析及优异等位基因挖掘. 保定:河北农业大学, 2010.
[23] 王曙光, 史雨刚, 史华伟 , 等. 春小麦光合特性与抗旱性的关系研究. 作物杂志, 2017(6):23-29.
doi: 10.16035/j.issn.1001-7283.2017.06.005
[24] 赵京岚 . 粗山羊草和普通小麦主要农艺性状与SSR标记的关联分析. 泰安:山东农业大学, 2014.
[25] 刘国祥 . 小麦抽穗期基因遗传作图. 雅安:四川农业大学, 2012.
[26] 朱玉磊, 王升星, 赵良侠 , 等. 以关联分析发掘小麦整穗发芽抗性基因分子标记. 作物学报, 2014,40(10):1725-1732.
doi: 10.3724/SP.J.1006.2014.01725
[27] 闫鹏娇 . 小麦K-CMS恢复系的遗传多样性研究及关联分析. 杨凌:西北农林科技大学, 2017.
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