Crops ›› 2025, Vol. 41 ›› Issue (2): 256-264.doi: 10.16035/j.issn.1001-7283.2025.02.035

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Effects of Exogenous Spermidine on the Interaction between Bemisia tabaci and Cucumber

Lu Yu(), Zhang Yanyan, Chen Haitao, Li Manxin, Bai Runʼe, Lei Caiyan()   

  1. College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, Henan, China
  • Received:2023-11-18 Revised:2024-03-17 Online:2025-04-15 Published:2025-04-16

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Abstract:

By using Q type Bemisia tabaci as experimental materials, effects of different concentrations of exogenous spermidine on the growth and development of the host plant and B.tabaci during the B.tabaci-host interaction process were determined, also the mechanism of the exogenous spermidine affecting the interaction between B.tabaci and the host were investigated at physiological level. The results showed that feeding of B.tabaci led to the decrease of plant height, leaf mass, photosynthetic rate, soluble protein content, polyphenol content and phenylalanine ammonia-lyase enzyme activity, the increase of soluble sugar content; whereas the treatment of spermidine reduced the damage of B.tabaci to the host plant, which was manifested in the promotion of growth and development of hosts, such as the enhancement of the photosynthetic capacity, the decrease of the soluble sugar content, the enhancement of the soluble protein content and polyphenol content and phenylalanine deaminase activity. Exogenous spermidine had impact on the growth and development of B.tabaci, manifested in low concentration of spermidine (0.5 mmol/L) reducing the number of eggs laid and the survival rate from egg to 1st-instar; high concentration of spermidine (2.0 mmol/L) significantly prolonged the developmental period of B.tabaci and reduced the survival rate of 3-4 instars. Therefore, during the process of B.tabaci damaging cucumbers, exogenous spermidine could promote plant growth and development of cucumbers, enhance photosynthetic capacity, alter plant nutritional characteristics, and improve defense ability. At the same time, it also has an adverse impact on the growth and development of B.tabaci, thus affecting the interaction between B.tabaci and host plants.

Key words: Spermidine, Bemisia tabaci-cucumber interaction, Growth and development, Physiological mechanism

Table 1

Experimental design"

处理
Treatment		 亚精胺浓度
Spd concentration (mmol/L)		 烟粉虱数量
Number of B.tabaci
CK0 0 0
CK1 0 25对
Spd1 0.5 25对
Spd2 1.0 25对
Spd3 2.0 25对

Fig.1

Plant height of cucumber treated with different concentrations of exogenous spermidine for different days (a) 5th day after inoculation; (b) 10th day after inoculation; (c) 15th day after inoculation; Different lowercase letters indicate significant differences at 0.05 level, the same below."

Fig.2

Fresh weight of cucumber leaves treated with different concentrations of exogenous spermidine for different days"

Fig.3

SPAD values of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.4

Ci of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.5

Tr of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.6

Pn of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.7

Contents of soluble sugar in cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.8

Contents of soluble protein of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.9

PAL activities of cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.10

Total phenol contents in cucumber treated with different concentrations of exogenous spermidine for different days"

Fig.11

Effects of different concentrations of exogenous spermidine on development period of B.tabaci at different stages"

Fig.12

Effects of different concentrations of exogenous spermidine on the body length and width of B.tabaci at different stages"

Fig.13

Effects of different concentrations of exogenous spermidine on laying eggs of B.tabaci"

Table 2

Effects of different concentrations of exogenous spermidine on survival rate of B.tabaci at different stages"

发育阶段
Developmental stage		 烟粉虱存活率B.tabaci survival rate (%) 差异显著性
Significance of difference
CK Spd1 Spd2 Spd3
卵-1龄Eggs-first-instar 0.99±0.01a 0.82±0.05b 0.86±0.06ab 0.88±0.05ab 0.096
1龄-2龄First-second-instar 0.91±0.08a 0.88±0.12a 0.98±0.02a 0.87±0.05a 0.776
2龄-3龄Second-third-instar 0.99±0.01a 0.98±0.04a 0.94±0.02a 0.97±0.03a 0.228
3龄-4龄Third-fourth-instar 0.97±0.03ab 0.89±0.07ab 0.98±0.01a 0.80±0.11b 0.153
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