Crops ›› 2018, Vol. 34 ›› Issue (4): 154-160.doi: 10.16035/j.issn.1001-7283.2018.04.026

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Characteristics of Cell Wall Degradation Enzyme Produced by Main Pathogenic Fusarium spp. in Potato Dry Rot

Chai Ying,Xu Yongqing,Fu Yao,Li Xiuyu,He Fumeng,Han Yingqi,Feng Zhe,Li Fenglan   

  1. College of Life Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2018-03-22 Revised:2018-06-18 Online:2018-08-20 Published:2018-08-23

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Abstract:

Dry rot is the main disease during the storage period of potato pits. Pathogens often invade the tubers through the mechanical abrasion of potato tuber and destroyed the parenchyma cells of the tuber to infect and spread. Therefore, it is of great significance to study the enzyme production characteristics of Fusarium. In this experiment, the characteristics of enzyme production by cell wall degrading enzymes in 5 Fusarium species and the effect of temperature on enzyme production characteristics were investigated by using Kexin 1 as the host material, test strains were Fusarium. sambucinum, F. avenaceum, F. trichothecioides, F. culmorum, and F. sporotriodides. The results showed that after inoculation of 5 species of Fusarium on potato tubers, amount of carboxymethyl cellulose (Cx), β-glucosidase, polygalacturonase (PG) and Methylgalacturonase (PMG) in all 5 treatments were all higher than that in control. The activity of cellulase was the highest in the late stage of infection, and the activity of pectinase was higher in the early stage of infection, and the cellulase activity was lower than that of pectinase. In an experiment on the effect of temperature on cell wall degrading enzymes produced by Fusarium in vivo, comparison with the control, the enzyme activities of Cx, PMG were higher at 25℃, the activity of β-glucosidase enzyme was higher at 20℃, the activity of PG was higher at both 20℃ and 25℃. The above results showed that Fusarium produced large amounts of cell wall degrading enzymes when infecting potato, cellulase activity was higher in the late stage of infection, pectinase activity was higher in the early stage of infection, and low temperature was not conducive to the production of fungal cell wall degrading enzymes.

Key words: Potato, Dry rot, Fusarium, In vivo culture, Characteristics of cell wall degradation enzyme, Culture conditions

Fig.1

The characteristics of cell wall degrading enzymes produced by dry rot pathogens in vivo"

Fig.2

The effects of culture temperature on activity of Cx produced by 5 Fusarium species"

Fig.3

The effects of culture temperature on activity of β-glucosidase produced by 5 Fusarium species"

Fig.4

The effects of culture temperature on activity of PG produced by 5 Furarium species"

Fig.5

The effects of culture temperature on activity of PMG produced by 5 Furarium species"

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