Crops ›› 2017, Vol. 33 ›› Issue (4): 134-142.doi: 10.16035/j.issn.1001-7283.2017.04.023

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Effects of PEG Stress on Drought Resistance at Seedling Stage of Adzuki Beans and the Establishment of Drought Resistance Identification System

Hao Xiyu1,Wang Hongdan2,Yin Zhichao3,Liang Jie1,Yin Fengxiang1,Hao Jianjun2   

  1. 1 Baicheng Academy of Agricultural Sciences,Baicheng 137000,Jilin,China
    2 School of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110161,Liaoning,China
    3 College of Plant Science,Jilin University,Changchun 130062,Jilin,China
  • Received:2017-04-12 Revised:2017-07-01 Online:2017-08-15 Published:2018-08-26
  • Contact: Jianjun Hao

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Abstract:

The drought-resistant cultivars including Baihong 3, Baihong 4, Baihong 5 and Jihong 352 as materials and PEG with different concentrations (5%, 10%, 20%) simulating water stress treatment in seedling stage of adzuki beans were used in this study. The function leaf on the third, sixth and ninth day was picked to determine drought resistance physiological indicators. The results showed that the indicators that could be used to identify the drought-resistance of adzuki bean were the SOD activity of 5% PEG treatment on the sixth day, the ABA content of 5% PEG treatment on the sixth day, the soluble sugar content of 10% PEG treatment on the sixth day, the electrical conductivity of 5% PEG treatment on the third day, the superoxide anion produce rate of 5% PEG treatment on the sixth day. The conclusion showed that Baihong 6 and Baihong 4 were both drought-resistance cultivars but Baihong 3 and Jihong 352 were less drought-resistance cultivars.

Key words: Adzuki bean, Seeding stage, Physiological index, Water stress, Drought-resistance

Table 1

Effects of PEG on SOD activity of different adzuki bean in seedling stage U/(g·h)"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 209.4671±2.8463 155.1778±11.1877* 194.7201±18.9780 200.8397±18.3817
6 204.4266±2.7343 240.8097±6.5492* 163.2722±29.2989* 267.7708±7.8024*
9 188.9510±17.1194 151.3179±20.5139* 168.0344±24.5121 144.7581±10.6570*
BH4 3 188.8430±0.3934 212.5512±6.2515* 234.2907±20.6791* 249.2962±10.6128**
6 185.0924±9.0046 248.5793±37.9914** 220.3664±1.4740* 157.6365±3.9680
9 231.5903±16.5768 204.4819±14.4766 204.8112±3.1630 248.7730±26.9031
BH6 3 152.1572±10.6956 144.6495±21.0837 131.0072±8.7254 146.1382±6.1430
6 86.5172±20.8143 171.4747±15.8480** 161.0846±9.5873** 126.3513±0.0000*
9 151.4920±2.0927 152.6208±2.3511 159.6148±5.8287 138.8985±21.0171
JH 3 142.1069±18.8383 90.1260±9.3786* 128.7792±18.6243 144.2592±15.7399
6 99.4462±1.9758 159.5897±15.3910* 146.8714±11.7565* 162.0526±21.8594*
9 129.2765±14.1346 166.3537±4.7483* 145.6508±25.4374 135.0340±25.7760

Table 2

Effects of PEG on POD activity of different adzuki bean in seedling stage U/(g·min)"

品种
Cultivar		 处理后天数(d)
The days after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 669.4607±32.0534 816.5082±35.7519** 844.5634±164.1243* 1 709.6340±32.1336**
6 944.6142±41.0331 1 458.2040±21.0823* 1 747.4420±55.5970** 1 717.4370±89.5941**
9 1 290.8950±48.5743 2 353.3730±60.8441** 1 822.2240±41.5332* 1 964.1530±58.1343**
BH4 3 713.5628±51.3608 813.1334±25.3332 1 209.3500±49.0023* 1 257.4260±193.0693*
6 1 109.4300±85.9260 1 383.7750±86.3887* 2 121.1230±175.9553** 2 103.9290±101.5647**
9 1 243.7140±49.9979 1 463.8031±2.6839** 1 651.9430±45.9024** 825.7638±27.3096**
BH6 3 672.3924±37.8112 1 075.4060±18.4207** 914.1072±66.2568** 1 483.5920±9.0981**
6 1 091.5310±49.2799 1 720.6720±60.8116** 1 766.7150±64.1127** 3 321.1050±118.5647**
9 1 279.3980±35.8979 2 406.4410±112.6528** 1 995.9270±76.0345** 1 915.5950±11.4213**
JH 3 790.6764±24.1842 897.2048±20.1691* 1 023.8000±41.5188** 1 533.7030±66.6677**
6 989.7014±54.0183 1 284.2290±110.6980* 2 083.7270±154.8725** 1 893.7760±87.6365**
9 1 222.1110±47.6711 1 340.1710±71.6815 2 174.8650±50.6280** 2 252.6170±136.5277**

Table 3

Effects of PEG on ABA content of different adzuki bean in seedling stage Ng/g·Fw"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 2 100.8500±148.2395 1 227.0800±3.0055** 1 655.0720±27.7170** 2 755.4330±333.3842*
6 1 347.1590±205.9270 778.0419±62.1796* 647.5509±39.4069* 1 485.8220±256.9520
9 1 611.3770±39.5107 2 440.8650±181.9251* 3 249.8250±575.2702* 3 601.0810±133.8049**
BH4 3 1 027.1840±150.0540 2 824.3520±9.9047** 1 130.6690±28.1881 2 607.3490±251.1899**
6 161.1756±24.7874 1 760.3010±99.8748** 637.9509±30.7409** 410.4254±119.2663*
9 1 258.3460±155.2239 2 769.6230±28.4783** 2 848.5640±311.0739** 3 077.1160±394.0654**
BH6 3 809.6446±18.2296 910.8683±3.8282** 762.1882±135.9778 2 657.2630±165.9192**
6 632.5677±227.1386 1 669.4410±247.5309** 1 205.5860±174.1826* 965.5097±209.9071
9 2 152.7610±79.7255 1 935.7820±50.0916 1 741.8820±319.1856 3 585.6020±287.2455**
JH 3 177.1649±5.0752 648.8794±1.4098** 958.4502±2.1028** 2 249.6610±11.9558**
6 351.3065±17.5732 1 619.1970±104.1080** 1 104.7560±90.3621** 983.8583±98.7873**
9 2 699.2700±27.6832 2 359.9380±225.1262 2 752.9020±150.6624 5 393.9120±754.3244**

Table 4

Effects of PEG on soluble sugar content of different adzuki bean in seedling stage %"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 2.7659±0.0352 3.2933±0.1098** 2.8417±0.1845 4.2388±0.0585**
6 2.3396±0.3172 2.4559±0.2408 3.2516±0.0983* 5.6213±0.0231**
9 4.4259±1.0191 3.8302±0.1202 3.1022±0.1895* 3.7034±0.1646
BH4 3 3.0655±0.1908 2.9458±0.1322 2.6507±0.0640* 3.4349±0.3005*
6 2.0265±0.0329 2.2695±0.6243 4.1954±0.0917** 6.4368±0.3261**
9 6.7551±1.5043 5.1418±0.3036* 4.4948±0.0871* 4.3183±0.3905*
BH6 3 3.1182±0.1908 2.5287±0.1038* 2.7812±0.0676* 2.9971±0.1902
6 2.0807±0.1788 2.6683±0.7653 3.3385±0.1384* 3.6029±0.0511*
9 3.3951±0.0224 3.0882±0.0796* 3.6827±0.2172* 3.7548±0.1629*
JH 3 2.8258±0.1199 2.7313±0.0606 3.4111±0.3140* 3.6270±0.1734*
6 2.3380±0.1846 2.7217±0.5721 3.2323±0.1516* 4.3383±0.3260**
9 3.4199±0.4897 4.3833±0.2201** 3.6036±0.1032 3.9701±0.0000*

Table 5

Effects of PEG on MDA content of different adzuki bean in seedling stage nmol/g"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 21.7605±0.8478 20.0989±0.8814 19.7361±1.4952 31.1533±1.1950**
6 31.8538±1.7966 30.0016±1.0715 26.3886±0.6059* 33.7942±1.0241
9 32.6741±2.3629 30.1034±4.3906 30.4844±1.1389 32.2909±1.3137
BH4 3 23.2361±1.5525 27.4701±2.3455* 17.8709±1.6886* 27.5671±0.9221*
6 29.1395±4.6508 21.3543±4.1138* 34.3946±1.9222 19.5738±2.8550*
9 27.2298±2.6975 25.0627±1.6173 26.6464±2.5250 26.8870±5.1578
BH6 3 24.8503±0.3068 25.2697±0.5852 29.3818±1.7590* 28.8575±1.5906*
6 29.6741±3.8452 24.4033±0.6927* 26.4576±1.2531 24.1422±1.2456*
9 29.9255±1.0973 29.1734±1.9957 28.3136±1.9809 25.3335±1.3580*
JH 3 29.6140±0.6444 30.5791±0.3185 37.4831±3.0639* 34.6157±7.0842
6 28.0198±0.7722 37.0707±1.9111** 32.5315±1.5175* 26.5262±0.5342
9 43.9681±1.7816 42.9565±0.2789 40.3243±1.6394* 41.2091±1.5021*

Table 6

Effects of PEG on relative electric conductivity of different adzuki bean in seedling stage %"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 10.8463±2.3192 6.7481±0.7416* 2.4209±0.8775** 3.8629±0.0286**
6 8.9370±2.6676 5.6232±1.2408 2.5567±4.0876** 5.8012±1.7782
9 6.0982±0.2475 5.3524±1.4591 7.2823±2.5553 10.1222±2.9359*
BH4 3 8.5270±0.6610 4.7164±1.1247* 3.0461±0.7166* 3.8522±1.8803*
6 11.6379±2.3651 4.0962±0.2142* 4.5183±0.5400* 24.9411±6.2024*
9 5.7667±1.6149 3.0853±0.7584 8.6574±4.6170 9.1369±0.8324
BH6 3 7.5184±0.2030 4.8940±0.6005* 2.7208±0.8222** 6.4064±1.6796
6 5.4259±0.6087 4.2957±1.2036 3.7440±0.2356* 14.9121±0.5540**
9 5.7393±2.2537 6.0981±0.7395 3.9793±0.7688 7.8652±2.1686
JH 3 9.0817±1.3497 6.4634±0.3537* 4.1015±0.6174** 6.4549±1.0952*
6 4.8730±1.3660 3.4424±0.5934 4.4968±0.3912 15.0880±0.1867*
9 5.7671±2.2777 4.5557±1.1841 4.8623±2.7127 9.0253±0.4168

Table 7

Effects of PEG on O2-· product rate of different adzuki bean in seedling stage nmol/(g·min)"

品种
Cultivar		 处理后天数(d)
The days of after treatment		 对照
CK		 5%PEG处理
5%PEG treatment		 10%PEG处理
10%PEG treatment		 20%PEG处理
20%PEG treatment
BH3 3 6.8862±0.2433 6.5553±0.1675 6.7508±0.2726 6.9182±0.1090
6 6.6025±0.0815 9.2417±0.3278* 10.3100±0.4340** 13.6347±0.2188**
9 7.8150±0.0900 11.2547±0.2417** 8.7460±0.1546** 11.2746±0.2053**
BH4 3 8.7751±0.4516 7.8602±1.0144 6.3302±0.0688* 7.4489±0.1797*
6 6.3709±0.0373 8.0933±0.2004** 14.3207±0.9337** 14.4628±0.2881**
9 8.6537±0.2253 9.2629±0.0814* 11.1661±0.2419** 17.5643±0.1044**
BH6 3 9.4807±0.2353 7.3479±0.4774** 6.0421±0.2452** 6.9413±0.1893**
6 7.5731±0.0705 8.9099±0.4593* 14.1430±0.6850** 14.3759±0.1438*
9 8.0628±0.0505 10.6628±0.1815** 9.0768±0.1424** 11.5582±0.3133**
JH 3 6.6864±0.1144 6.5879±0.4716 5.7940±0.2848* 5.9760±0.0936*
6 6.6782±0.1120 8.8939±0.1328* 11.7599±0.6501** 14.3341±0.2086**
9 10.4811±0.1990 9.5870±0.1604** 13.0856±0.0290** 9.1546±0.0743**

Table 8

Changes of the physiological indexes of the resistance to drought compared with the control in the seedling stage of adzuki beans"

指标Index 处理Treatment BH3 BH4 BH6 JH
SOD活性SOD activity CK 204.43 185.09 86.52 99.45
处理Treatment 240.81 248.58 171.47 159.59
△% 17.80 34.30 98.19 60.47
ABA含量ABA content CK 1347.16 161.18 632.57 351.31
处理Treatment 778.04 1 760.30 1 669.44 1 619.20
△% -42.25 992.13 163.91 360.90
可溶性糖含量Soluble sugar content CK 2.34 2.03 2.08 2.34
处理Treatment 3.25 4.20 3.34 3.23
△% 38.89 106.90 60.58 38.03
相对电导率Relative electric conductivity CK 10.85 8.53 7.52 9.08
处理Treatment 6.75 4.72 4.90 6.46
△% -37.79 -44.67 -19.70 -28.85
O2-·产生速率O2-·product rate CK 6.60 6.37 7.57 6.68
处理Treatment 9.24 8.09 8.91 8.89
△% 40.00 27.00 17.70 33.08

Table 9

The drought resistance level of adzuki bean in seedling stage"

品种
Cultivar		 SOD活性
SOD activity		 ABA含量
ABA content		 可溶性糖含量
Contents of soluble sugar		 相对电导率
Relative conductivity		 O2-·产生速率
O2-·producing rate		 抗旱级数
Drought resisting series
BH3 1 1 2 3 1 8
BH4 2 4 4 4 3 17
BH6 4 2 3 1 4 14
JH 3 3 1 2 2 11
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