Crops ›› 2019, Vol. 35 ›› Issue (5): 22-27.doi: 10.16035/j.issn.1001-7283.2019.05.004

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Genetic Diversity Analysis and Code Classification Based on DUS Testing in Sunflower

Wang Yongxing1,2,Shan Feibiao1,2,Yan Wenzhi1,2,Du Ruixia1,2,Yang Qinfang1,2,Liu Chunhui1,2,Bai Lihua1   

  1. 1 Bayannur Academy of Agricultural and Animal Sciences, Linhe 015000, Inner Mongolia, China
    2 Bayannur Sub-Center for New Plant Variety Tests, Ministry of Agriculture and Rural Affairs, Linhe 015000, Inner Mongolia, China
  • Received:2019-01-29 Revised:2019-07-10 Online:2019-10-15 Published:2019-11-07
  • Contact: Feibiao Shan

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Abstract: This experiment with 253 sunflower germplasm resources from all parts of the country carried correlation, regression, genetic diversity analysis which provided reference for the development, utilization and evaluation of sunflower germplasm resources. The quantitative traits were classified by the method of double standard deviation, and the scientific nature of code classification was discussed by frequency analysis, which laid the basis for the revision of sunflower DUS testing guideline. Through correlation and regression analysis, it was found that the leaf size had a very significant correlation with the leaf length and leaf width, and the seed size had very significant correlation with the seed length and seed width, and there had a certain linear functional relationship between them. According to character code classification and frequency analysis, it was found that the classification of bract density, ligulate flower density, leaf length and seed thickness was relatively small, and the data were relatively concentrated, which was not conducive to distinguishing different varieties. Although seed length, seed width and seed size were classified much, they did not conform to the normal distribution, it showed that the code classification was not scientific and reasonable. However, the data distribution of leaves of main stem, leaf width, leaf size, disc diameter and plant height conformed to the normal distribution, code classification is more scientific and useful to distinguish different varieties. The main reason for the unreasonable code classification is the result of manual selection, which is also affected by poor awareness of variety protection and imperfect laws and regulations.

Key words: DUS testing, Sunflower, Genetic diversity, Code classification

Table 1

Quantitative trait description"

性状Trait 平均
Mean		 中位数
Middle number		 标准差
SD		 最小值
Min		 最大值
Max		 变异系数
CV (%)
主茎叶数Leaves of main stem 32.84 32.10 2.17 22.40 54.10 3.26
舌状花密度(个/盘) Ligulate flower density (number/disk) 32.38 44.22 5.47 22.41 86.74 7.29
苞叶密度(个/盘) Bract density (number/disk) 46.25 63.40 5.34 36.55 94.31 11.55
叶长Leaf length (mm) 313.62 315.70 39.30 197.80 405.00 12.53
叶宽Leaf width (mm) 311.87 312.00 26.81 179.50 457.00 4.90
叶片大小Leaf size (mm2) 994.43 991.41 78.35 362.76 1 679.80 7.88
花盘直径Disc diameter (mm) 225.47 226.65 11.68 98.00 327.20 5.18
株高Plant height (cm) 186.31 177.90 12.71 76.53 332.71 6.82
瘦果长度Seed length (mm) 20.45 22.14 0.96 9.48 26.27 4.69
瘦果宽度Seed width (mm) 8.26 8.56 0.49 3.88 11.08 5.93
瘦果大小Seed size (mm2) 174.64 188.97 11.38 38.61 280.55 6.52
瘦果厚度Seed thickness (mm) 4.19 4.16 0.47 2.51 5.82 11.26

Table 2

Correlation analysis of quantative characters"

性状
Trait		 主茎叶数
Leaves of
main stem		 舌状花密度
Ligulate flower
ensity		 苞叶密度
Bract
density		 叶长
Leaf
length		 叶宽
Leaf
width		 叶片大小
Leaf size		 花盘直径
Disc diameter		 株高
Plant height		 瘦果长度
Seed length		 瘦果宽度
Seed width		 瘦果大小
Seed size
舌状花密度 0.13*
苞叶密度 0.37** -0.53**
叶长 0.16* -0.03 -0.05
叶宽 0.08 0.05 -0.01 0.84**
叶片大小 0.10 0.05 -0.01 0.86** 0.87**
花盘直径 0.32** 0.18** -0.20** 0.53** 0.50** 0.49**
株高 0.64** 0.18** -0.24** 0.33** 0.31** 0.30** 0.31**
瘦果长度 0.20** -0.05 -0.13* 0.39** 0.29** 0.34** 0.34** 0.37**
瘦果宽度 0.18** 0.04 -0.03 0.45** 0.36** 0.39** 0.51** 0.33** 0.88**
瘦果大小 0.21** 0.01 -0.07 0.42** 0.33** 0.37** 0.45** 0.37** 0.96** 0.96**
瘦果厚度 0.04 0.07 -0.06 0.42** 0.35** 0.38** 0.55** 0.04 0.44** 0.69** 0.57**

Table 3

Abstract of regression analysis model"

性状Trait R R2 调整后R2 Adjusted R2 标准估算的误差Error in standard estimates
叶片大小Leaf size 0.902 0.814 0.812 10.6300
瘦果大小Seed size 0.985 0.970 0.969 9.3975

Table 4

Variance analysis"

性状Trait 模型Model 平方和SS 自由度DF 均方MS F 显著性Significance
叶片大小Leaf size 回归Regression 10 940 983.380 2 5 470 491.690 545.816 0.000
残差Residual 2 505 650.267 250 10 022.601
总计Total 13 446 633.640 252
瘦果大小Seed size 回归Regression 706 634.884 2 353 317.442 4 000.741 0.000
残差Residual 22 078.251 250 88.313
总计Total 728 713.136 252

Table 5

Regression equation coefficient"

性状
Trait		 模型
Model		 未标准化系数
Unnormalized coefficient		 标准误
Standard error		 标准化系数
Normalized coefficient		 t 显著性
Significance
叶片大小Leaf size 常量Constant -730.372 53.932 -13.542 0.000
叶长X1 Leaf length X1 2.896 0.315 0.464 9.200 0.000
叶宽X2 Leaf width X2 2.621 0.278 0.476 9.444 0.000
瘦果大小Seed size 常量Constant -121.054 3.684 -32.863 0.000
瘦果长度x1 Seed length x1 6.558 0.299 0.517 21.957 0.000
瘦果宽度x2 Seed width x2 19.561 0.926 0.497 21.114 0.000

Table 6

Classification of 12 quantitative traits"

性状Trait 平均Mean 最小值Min 最大值Max 极差Range (Max-Min) 级数1 Step 1 级数2 Step 2 级差Range
主茎叶数
Leaves of main stem		 32.84 22.40 54.10 31.70 7.30 7 4.53
舌状花密度(个/盘)
Ligulate flower density (number/disk)		 32.38 22.41 86.74 64.33 5.88 5 12.87
苞叶密度(个/盘)
Bract density (number/disk)		 46.25 36.55 94.31 57.76 5.41 5 11.55
叶长Leaf length (mm) 313.62 197.80 405.00 207.20 7.67 3 69.07
叶宽Leaf width (mm) 311.87 179.50 457.00 277.50 5.18 5 55.50
叶片大小Leaf size (mm2) 994.43 362.76 1 679.80 1 317.04 8.40 7 188.15
花盘直径Disc diameter (mm) 225.47 98.00 327.20 229.20 9.81 9 25.47
株高Plant height (cm) 186.31 76.53 332.71 256.18 10.08 9 28.46
瘦果长度Seed length (mm) 20.45 9.48 26.27 16.80 8.75 7 2.40
瘦果宽度Seed width (mm) 8.26 3.88 11.08 7.20 7.34 7 1.03
瘦果大小Seed size (mm2) 174.64 38.61 280.55 241.93 10.63 9 26.88
瘦果厚度Seed thickness (mm) 4.90 2.51 5.82 3.31 3.51 3 1.10

Fig.1

Frequency distribution of main quantitative characters of sunflower"

Table 7

Genetic diversity index of 12 quantitative traits"

分级
Classification		 主茎叶数
Leaves of main stem		 舌状花密度
Ligulate flower density		 苞叶密度
Bract density		 叶长
Leaf length		 叶宽
Leaf width		 叶片大小
Leaf size
1 - - - - - -
2 0.18 - - - - 0.09
3 0.33 0.04 0.02 - 0.13 0.20
4 0.32 0.36 0.34 0.29 0.32 0.34
5 0.34 0.34 0.34 0.25 0.34 0.36
6 0.33 0.30 0.32 0.28 0.33 0.33
7 0.14 0.04 0.08 - 0.11 0.23
8 0.09 - - - - 0.08
9 - - - - - -
多样性指数Diversity index 1.74 1.08 1.10 0.81 1.22 1.63
分级
Classification		 花盘直径
Disc diameter		 株高
Plant height		 瘦果长度
Seed length		 瘦果宽度
Seed width		 瘦果大小
Seed size		 瘦果厚度
Seed thickness
1 0.14 0.02 - - 0.29 -
2 0.05 0.04 0.30 0.14 0.08 -
3 0.21 0.20 0.07 0.24 0.04 -
4 0.33 0.35 0.26 0.23 0.30 0.17
5 0.36 0.36 0.34 0.34 0.36 0.16
6 0.33 0.28 0.30 0.33 0.35 0.25
7 0.26 0.27 0.04 0.02 0.22 -
8 0.08 0.16 - 0.02 0.04 -
9 0.02 0.14 - - 0.00 -
多样性指数Diversity index 1.77 1.81 1.31 1.34 1.67 0.58
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