Crops ›› 2021, Vol. 37 ›› Issue (1): 82-89.doi: 10.16035/j.issn.1001-7283.2021.01.012

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Effects of PEG Simulated Drought Stress on Seedling Morphology and Physiological Characteristics of Different Drought-Resistance Maize Varieties

Yang Juan1,2(), Jiang Yangming3, Zhou Fang1, Zhang Jun1, Luo Haideng1, Tian Shanjun1()   

  1. 1College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China
    2Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, Hainan, China
    3State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, Guizhou, China
  • Received:2020-04-15 Revised:2020-12-21 Online:2021-02-15 Published:2021-02-23
  • Contact: Tian Shanjun E-mail:1395975299@qq.com;tsjscau@163.com

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Abstract:

In order to explore the response mechanism of maize (Zea mays L.) to water deficit at seedling stage, this study used two maize varieties with significant differences in drought resistance as materials, and used PEG-6000 solution to simulate drought stress of different intensities, and the morphological, physiological and biochemical characteristics of maize seedlings were analyzed. The results showed that, after drought stress, the plant height, leaf area, root length, and root weight of Chengdan 30 with strong drought-resistance showed an increasing trend, while Jinyu 306 with weak drought-resistance showed a downward trend. Drought stress increased the shoot dry weight, root weight, and dry matter weight of Chengdan 30, while the yield of each organism of Jinyu 306 were gradually decreased. Drought stress led to an increase in malondialdehyde content and a decrease in cell membrane stability. Drought stress reduced the chlorophyll a/b ratio and chlorophyll content of maize, and increased carotenoid content. The soluble sugar and soluble protein content of Chengdan 30 did not change significantly after drought stress, but Jinyu 306 did not change significantly until the later period. Proline contents of Chengdan 30 and Jinyu 306 both increased significantly after drought stress. Drought stress increased the activities of superoxide dismutase, peroxidase and catalase in two maize varieties. These results indicate that the drought resistance mechanisms of maize with different drought-resistant are different.

Key words: Maize, Seedling stage, Drought stress, Response mechanism

Table 1

Morphological indicators of two maize varieties under drought stress condition"

处理
Treatment		 株高
Plant height (cm)		 叶面积(cm2/株)
Leaf area (cm2/plant)		 根长(cm/株)
Root length(cm/plant)		 根体积(cm3/株)
Root volume (cm3/plant)
48h 144h 48h 144h 48h 144h 48h 144h
C-CK 37.29Bb 38.94Bc 99.43Bb 102.57Bb 32.13Bb 33.22Ab 3.28Bc 5.96Bb
C-T1 43.08Aa 51.32Aa 116.58Aa 133.09Aa 37.12Aa 35.87Aab 5.83Aa 7.08Aa
C-T2 43.25Aa 47.54Ab 107.53ABab 106.86Bb 38.97Aa 36.32Aa 4.28Bb 6.40ABab
J-CK 48.33a 51.08Ab 151.38a 177.83Aa 35.18a 37.23a 9.30Aa 7.30a
J-T1 51.22a 55.32Aa 149.26a 159.95Bb 35.44a 38.96a 5.48Bb 7.62a
J-T2 49.27a 50.71Aab 146.52a 147.56Cc 33.21a 35.36a 6.13Bb 7.97a

Table 2

Biological yields per plant of two maize varieties under drought stress condition"

处理
Treatment		 地上部干重Shoot dry weight (g) 根重Root weight (g) 干物重Dry matter weight (g) 根冠比Shoot/Root (%)
48h 144h 48h 144h 48h 144h 48h 144h
C-CK 0.48Bb 0.72Aab 0.22Bc 0.26Cc 0.70Bb 1.06Bb 47.80a 48.64a
C-T1 0.68Aa 0.85Aa 0.31Aa 0.40Aa 1.00Aa 1.26Aa 48.78a 43.84a
C-T2 0.53Bb 0.70Ab 0.27Bb 0.33Bb 0.81Bb 1.02Bb 51.24a 43.45a
J-CK 0.84Aa 0.86a 0.45Aa 0.48a 1.27Aa 1.34Aa 56.37a 53.60a
J-T1 0.73Aab 0.93a 0.35Bb 0.46a 1.08Bb 1.40Aa 55.07a 48.05a
J-T2 0.69Ab 0.83a 0.35Bb 0.47a 1.03Bb 1.13Bb 57.52a 48.26a

Fig.1

Chlorophyll a/b, chlorophyll content and carotenoid content of two maize varieties under drought stress condition Different lowercase letters indicate significant difference (P < 0.05), and different capital letters indicate significant difference (P < 0.01). The same below"

Fig.2

MDA content and cell membrane stability of two maize varieties under drought stress condition"

Table 3

The contents of soluble protein, soluble sugar, and proline of two maize varieties under drought stress condition μg/g"

处理
Treatment		 可溶性蛋白Soluble protein 可溶性糖Soluble sugar 脯氨酸Proline
48h 144h 48h 144h 48h 144h
C-CK 1.08a 0.91a 4.40a 5.38Aa 6.73Bc 10.10Cc
C-T1 1.04a 0.94a 4.35a 4.16Ab 38.71Ab 78.69Bb
C-T2 1.12a 0.86a 4.77a 5.63Aa 47.97Aa 104.78Aa
J-CK 1.44Aab 0.80Cc 4.80Aa 7.46Aa 7.57Cc 11.36Cc
J-T1 1.55Aa 0.98Aa 4.25Aab 7.60Aa 21.46Bb 47.13Bb
J-T2 1.05Ab 0.85Bb 3.85Ab 4.48Bb 34.93Aa 62.70Aa

Table 4

SOD, POD and CAT activities of two maize varieties under drought stress condition"

处理
Treatment		 CAT [nmol/(min·g)] POD [nmol/(min·g)] SOD (U/g)
48h 144h 48h 144h 48h 144h
C-CK 33.90Cc 30.57Cc 146.67Cc 86.67Cc 9.33Cc 29.00Cc
C-T1 43.63Bb 40.27Bb 222.22Bb 228.89Bb 11.90Bb 36.77Bb
C-T2 54.97Aa 50.65Aa 308.89Aa 362.22Aa 17.02Aa 63.93Aa
J-CK 30.13Bb 30.70a 88.89Bc 120.00Cc 6.89Ab 14.91Cc
J-T1 33.26Bb 32.55a 126.67Bb 208.89Bb 8.08Ab 24.70Bb
J-T2 44.40Aa 29.64a 197.78Aa 351.11Aa 12.67Aa 33.20Aa
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