Crops ›› 2021, Vol. 37 ›› Issue (1): 74-81.doi: 10.16035/j.issn.1001-7283.2021.01.011

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Effects of Simulated Drought Stress on the Physiological Indexes and Contents of Active Components of Potted "Chuandanshen 1"

Deng Wanyue(), Leng Qiuyan, Yang Zaijun, Yu Yan, Wu Yichao()   

  1. College of Life Science, China West Normal University, Nanchong 637002, Sichuan, China
  • Received:2020-04-15 Revised:2020-07-02 Online:2021-02-15 Published:2021-02-23
  • Contact: Wu Yichao E-mail:1149017145@qq.com;chaoyue-wu@qq.com

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Abstract:

In this study, polyethylene glycol (PEG-6000) was used to simulate long-term drought stress on potted 'Chuandanshen 1' (CDS-1) for investigating the effects of drought stress on the growth, chlorophyll content, physiological indicators, and the content of main active components. The results were as follows: Drought stress significantly reduced the chlorophyll content in the leaves. Under drought stress, the contents of soluble protein, soluble sugar and proline in leaves showed a significant increasing trend with the increase of drought. MDA content increased significantly under middle and severe drought stress. Antioxidant enzyme activity increased with the increasing of drought stress. HPLC analysis of active components showed that phenolic acid and tanshinone content in the root increased after treating with 50-150g/L of PEG-6000. The content of rosmarinic acid and salvianolic acid A were the highest when treated with 150g/L, which was 145% and 175% of CK. The content of tanshinones was the highest when treated with 50g/L PEG-6000, and increased more than 60% compared with CK. Overall, the results demonstrated that CDS-1 showed adaptive changes under drought stress and had a certain drought-resistant ability. The activity of antioxidant enzyme and the contents of main active components of CDS-1 increased, which improved its antioxidant and disease-prevention ability to adapt the drought environment. Light and middle drought stress (50-100g/L PEG-6000) are beneficial to the accumulation of phenolic acid and tanshinones in CDS-1. In actual production, scientific control of water can be used to ensure and improve the quality of medicinal material CDS-1.

Key words: Chuandanshen 1, Drought stress, Physiological index, HPLC, Active components

Table 1

Different concentrations of PEG-6000 simulate drought stress treatments"

PEG浓度
PEG concentration(g/L)		 水势梯度
Water potential gradient (MPa)		 干旱程度
Degree of drought
0 (CK) 0 –
50 -0.1 轻度
100 -0.4 中度
150 -0.5 中度
200 -0.7 重度

Table 2

Standard curves of six active components of CDS-1"

化合物Compound 回归方程Regression equation 相关系数Correlation coefficient (r) 线性范围Linear range (mg/mL)
迷迭香酸Rosmarinic acid Y=4720.4X+30.738 0.9982 0.0025~0.0625
丹酚酸B Salvianolic acid B Y=13362X+12.023 0.9998 0.0200~0.2000
丹酚酸A Salvianolic acid A Y=8580.5X+41.569 0.9970 0.0025~0.0625
隐丹参酮Cryptotanshinone Y=61908X-3.0334 1 0.0005~0.0125
丹参酮ⅠTanshinone Ⅰ Y=150729X+0.5474 1 0.0005~0.0125
丹参酮ⅡA Tanshinone ⅡA Y=171690X-0.0514 1 0.00037~0.00555

Fig.1

Morphology of CDS-1 under PEG-6000 simulated drought stress with different concentrations"

Table 3

Chlorophyll content in leaves of CDS-1 under PEG-6000 simulated drought stress"

PEG-6000浓度
PEG-6000
concentration
(g/L)		 叶绿素含量
Chlorophyll content (mg/g)		 叶绿素a/b
Chl a/Chl b
Chl a Chl b 合计
Total
0 (CK) 0.88±0.10a 0.49±0.06a 1.37±0.16a 1.82±0.12c
50 0.73±0.04b 0.38±0.02b 1.11±0.06b 1.89±0.09bc
100 0.72±0.04b 0.33±0.03bc 1.05±0.07b 2.15±0.30abc
150 0.70±0.04b 0.31±0.02bc 1.01±0.06b 2.22±0.11ab
200 0.66±0.07b 0.29±0.04c 0.95±0.11b 2.29±0.27a

Fig.2

The soluble protein, soluble sugar and proline content in leaves of CDS-1 under PEG-6000 simulated drought stress"

Fig.3

MDA content, and the activities of SOD, POD and CAT in leaves of CDS-1 under PEG-6000 simulated drought stress"

Fig. 4

The HPLC chromatography of six active components for CDS-1 under PEG-6000 simulated drought stress 1. Rosmarinic acid, 2. Salvianolic acid B, 3. Salvianolic acid A, 4. Cryptptanshinone, 5. Tanshinone Ⅰ, 6. Tanshinone ⅡA"

Table 4

The contents of six active components of CDS-1 under PEG-6000 simulated drought stress mg/g"

PEG-6000浓度
PEG-6000 concentration (g/L)		 迷迭香酸
Rosmarinic
acid		 丹酚酸B
Salvianolic
acid B		 丹酚酸A
Salvianolic
acid A		 隐丹参酮
Cryptptanshinone		 丹参酮Ⅰ
TanshinoneⅠ		 丹参酮ⅡA
TanshinoneⅡA		 总丹参酮
Total tanshinone
0 (CK) 5.54±0.25c 34.44±1.77a 0.53±0.16b 0.05±0.01c 0.14±0.01b 0.19±0.01c 0.38±0.01b
50 6.00±0.31c 31.60±1.31bc 0.54±0.12b 0.09±0.00a 0.23±0.01a 0.32±0.02a 0.64±0.03a
100 7.64±0.26a 30.00±1.48c 0.58±0.09b 0.07±0.01b 0.24±0.02a 0.28±0.02b 0.59±0.01a
150 8.03±0.36a 34.44±1.12a 0.93±0.17a 0.01±0.00d 0.09±0.00c 0.07±0.01d 0.17±0.01c
200 6.84±0.27b 32.68±0.96ab 0.41±0.09b 0.05±0.01c 0.15±0.03b 0.20±0.02c 0.40±0.05b
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