Crops ›› 2025, Vol. 41 ›› Issue (1): 194-201.doi: 10.16035/j.issn.1001-7283.2025.01.024

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Effects of Seed Soaking with Salicylic Acid on Seed Germination and Seedling Growth of Quinoa under Salt Stress

Jing Maoya1(), Zhang Ziyu1, Zhang Meng1, He Jiamin1, Yan Fanfan1, Gao Yanmei1(), Zhang Yongqing1,2()   

  1. 1College of Life Science, Shanxi Normal University, Taiyuan 030031, Shanxi, China
    2College of Geography Science, Shanxi Normal University, Taiyuan 030031, Shanxi, China
  • Received:2023-12-20 Revised:2024-04-05 Online:2025-02-15 Published:2025-02-12

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Abstract:

In order to investigate the alleviating mechanism of seed soaking with salicylic acid on salt-induced inhibition of seed germination and seedling growth in quinoa (Chenopodium quinoa Willd.), Longli 1 (salt- sensitive) and Longli 4 (salt-tolerant) were used as experimental materials to study the effects of different concentrations (0, 0.10, 0.25, 0.50, 1.00 mmol/L) of salicylic acid on seed germination, seedling growth, and physiology under 200 mmol/L NaCl moderate salt stress. The results showed that the seed germination rates of both quinoa varieties significantly decreased under salt stress, and the growth of seedlings were inhibited. After treatmenting with salicylic acid, the negative effects caused by salt stress were significantly alleviated, the germination rates, germination vigor, and germination indexes were significantly increased after soaking treatments, and effectively promoted seed germination. Furthermore, the seedling growth parameters of two quinoa varieties, aboveground dry weight, antioxidant enzyme activity, osmoregulatory substances, chlorophyll content, and root activity all exhibited a trend of first rising and then falling as the salicylic acid soaking concentration increased. The MDA content first decreased and then increased with the increasing of salicylic acid soaking concentration. It was discovered that the optimal concentration of 0.25 mmol/L salicylic acid for seed soaking had the best regulatory effect on Longli 1 under salt stress, while the optimal concentration of 0.10 mmol/L salicylic acid for seed soaking had the best regulatory effect on Longli 4 under salt stress.

Key words: Quinoa, Seed soaking with salicylic acid, Salt stress, Seed germination, Seedling growth

Table 1

The effects of salicylic acid soaking seeds on the germination of quinoa seeds under salt stress"

品种
Variety		 处理
Treatment		 发芽率
Germination
rate (%)		 发芽势
Germination
vigor (%)		 发芽指数
Germination
index
L1 CK 92.2±0.0ab 88.7±0.0a 68.2±3.6a
S1 85.0±0.0de 82.3±0.0b 59.3±4.4a
S2 88.9±0.0bc 86.7±0.0a 61.8±3.9a
S3 92.7±0.0a 90.7±0.0a 73.6±5.9a
S4 87.8±0.0cd 87.3±0.0a 60.8±1.6a
S5 83.9±0.0e 81.0±0.0b 58.3±1.4a
L4 CK 95.6±0.0a 92.7±0.0a 72.2±1.6a
S1 88.9±0.0b 87.7±0.0b 60.2±1.1b
S2 96.1±0.0a 93.3±0.0a 72.7±4.6a
S3 91.7±0.0b 89.3±0.0b 68.5±1.9ab
S4 88.9±0.0b 87.3±0.0b 59.0±0.6b
S5 80.0±0.0c 79.0±0.0c 47.9±1.4c

Table 2

Effects of salicylic acid soaking seeds on plant height and root length of quinoa under salt stress cm"

品种Variety 处理Treatment 株高Plant height 根长Root length
L1 CK 20.5±0.3a 11.3±0.4b
S1 17.1±0.7b 13.3±0.3ab
S2 20.1±0.3a 14.5±0.5a
S3 21.4±0.6a 15.7±0.3a
S4 19.3±0.5ab 14.4±1.1a
S5 17.1±1.0b 11.6±0.3b
L4 CK 23.0±0.9a 8.3±0.2d
S1 18.3±0.7b 10.7±0.3c
S2 23.3±0.6a 13.6±0.5a
S3 21.2±0.7ab 12.2±0.2b
S4 18.1±1.1b 10.5±0.4c
S5 12.5±0.6c 7.1±0.4d

Fig.1

Effects of salicylic acid soaking seeds on the aboveground dry weight of quinoa under salt stress Different lowercase letters indicate significant difference at P < 0.05 level. The same below."

Fig.2

Effects of salicylic acid soaking seeds on antioxidant enzyme activity of quinoa seedlings under salt stress"

Fig.3

Effects of salicylic acid soaking seeds on MDA content of quinoa seedlings under salt stress"

Fig.4

Effects of salicylic acid soaking seeds on osmotic regulating substances of quinoa seedlings under salt stress"

Fig.5

Effects of salicylic acid soaking seeds on chlorophyll content in leaves of quinoa seedlings under salt stress"

Fig.6

Effects of salicylic acid soaking seeds on root activity of quinoa seedlings under salt stress"

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