Crops ›› 2016, Vol. 32 ›› Issue (5): 13-18.doi: 10.16035/j.issn.1001-7283.2016.05.003

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SSR Analysis of Winter Wheat Lines of Beijing Regional Trials from 2009 to 2015

Liu Lihua1,Pang Binshuang1,Li Hongbo1,Liu Yangna1,Fu Deping2,Wang Na1,Zhao Changping1   

  1. 1 Beijing Engineering and Technique Research Center for Hybrid Wheat,Beijing Academy of Agriculture and Forestry Sciences/The Municipal Key Laboratory of the Molecular Genetics of Hybrid Wheat,Beijing 100097,China
    2 Beijing Seed Management Station,Beijing 100026,China
  • Received:2016-07-12 Revised:2016-08-22 Online:2016-10-15 Published:2018-08-26
  • Contact: Changping Zhao

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Abstract:

SSR Analysis of 130 winter wheat lines from Beijing regional trials from 2009 to 2015 could provide a theoretical basis for the wheat variety management and breeding. The SSR fingerprint detection results showed that thirty wheat lines had poor uniformity and stability, twenty-eight wheat lines were similar with regional trials lines or varieties, two wheat lines were different between two years. There were fewer problem wheat lines in 2013-2015. The genetic diversity showed that there were different degrees of genetic variations among all the regional trials lines, but the high frequency use of the backbone parents reduced the genetic diversity of the population. There was no significant change in genetic diversity among different years. In the future, in order to improve the genetic diversity and broaden the genetic background of winter wheat varieties in Beijing, the breeding resources, breeding patterns and breeding direction should be changed.

Key words: Beijing regional trials, Winter wheat, SSR fingerprint, Line

Table 1

Information of 21 pairs of primers"

引物
Primer		 染色体
Chromosome		 退火温度(℃)
Annealing temperature		 引物序列（5′-3′）
Primer sequence
cwm65
 1A
 65
 F：TCATTGGTGTCATCCCTCGTGT
R：GAATAATGCCTTGACCCTGGAC
barc80
 1BL
 65
 F：GCGAATTAGCATCTGCATCTGTTTGAG
R：CGGTCAACCAACTACTGCACAAC
cfd72
 1DL
 60
 F：CTCCTTGGAATCTCACCGAA
R：TCCTTGGGAATATGCCTCCT
gwm294
 2AL
 55
 F：GGATTGGAGTTAAGAGAGAACCG
R：GCAGAGTGATCAATGCCAGA
gwm429
 2BS
 55
 F：TTGTACATTAAGTTCCCATTA
R：TTTAAGGACCTACATGACAC
gwm261
 2DS
 55
 F：CTCCCTGTACGCCTAAGGC
R：CTCGCGCTACTAGCCATTG
gwm155
 3AL
 55
 F：CAATCATTTCCCCCTCCC
R：AATCATTGGAAATCCATATGCC
gwm285
 3BS
 65
 F：ATGACCCTTCTGCCAAACAC
R：ATCGACCGGGATCTAGCC
gdm72
 3DS
 55
 F：TGGTTTTCTCGAGCATTCAA
R：TGCAACGATGAAGACCAGAA
gwm610
 4AS
 65
 F：CTGCCTTCTCCATGGTTTGT
R：AATGGCCAAAGGTTATGAAGG
ksum62
 4B
 60
 F：GGAGAGGATAGGCACAGGAC
R：GAGAGCAGAGGGAGCTATGG
barc91
 4DL
 55
 F：TTCCCATAACGCCGATAGTA
R：GCGTTTAATATTAGCTTCAAGATCAT
cwem40
 5A
 58
 F：TAGCACCAGGCTTGACCAGT
R：GGACCAAAGCCAAAAACAAA
gwm67
 5BL
 60
 F：ACCACACAAACAAGGTAAGCG
R：CAACCCTCTTAATTTTGTTGGG
cfd29
 5DL
 65
 F：GGTTGTCAGGCAGGATATTTG
R：TATTGATAGATCAGGGCGCA
gwm334
 6AS
 55
 F：AATTTCAAAAAGGAGAGAGA
R：AACATGTGTTTTTAGCTATC
barc198
 6BS
 55
 F：CGCTGAAAAGAAGTGCCGCATTATGA
R：CGCTGCCTTTTCTGGATTGCTTGTCA
cfd76
 6DL
 65
 F：GCAATTTCACACGCGACTTA
R：CGCTCGACAACATGACACTT
cfa2028
 7AS
 55
 F：TGGGTATGAAAGGCTGAAGG
R：ATCGCGACTATTCAACGCTT
gwm333
 7BS
 60
 F：GCCCGGTCATGTAAAACG
R：TTTCAGTTTGCGTTAAGCTTTG
gwm437
 7DL
 55
 F：GATCAAGACTTTTGTATCTCTC
R：GATGTCCAACAGTTAGCTTA

Table 2

The number of the problematic wheat lines that were detected in Beijing"

年份
Year		 一致性和稳定性
Uniformity and stability		 疑似品系
Suspected line		 真实性
Genuineness
2009 3 8 0
2010 6 7 0
2011 10 2 1
2012 5 5 0
2013 1 1 0
2014 2 2 1
2015 3 3 0
合计Total 30 28 2

Fig.1

Genetic diversity comparison of wheat national regional trial in different years"

Fig.2

UPGMA cluster diagram of 114 wheat lines based on Nei’s genetic distance （1-114 were the codes of wheat lines）"

[1] 王立新, 常利芳, 李宏博 , 等. 小麦区试品系DUS测试的分子标记. 作物学报, 2010,36(7):1114-1125.
doi: 10.3724/SP.J.1006.2010.01114
[2] 刘丽华, 庞斌双, 刘阳娜 , 等. 2009-2014年国家冬小麦区域试验品系的遗传多样性及群体结构分析. 麦类作物学报, 2016,26(2):165-171.
doi: 10.7606/j.issn.1009-1041.2016.02.06
[3] Wang L X, Liu L H, Zhang F T , et al. Detecting seed purity of wheat varieties using microsatellite markers based on eliminating the influence of non-homozygous loci. Seed Science & Technology, 2014,42:1-21.
[4] Wang L X, Pang B S, Liu L H , et al. Assessment of wheat variety uniformity using SSR Markers. Molecular Plant Breeding, 2015,6(21):1-17.
doi: 10.1057/9781137496591_1
[5] Wang L X, Li H B, Gu T C , et al. Assessment of wheat variety stability using SSR markers. Euphytica, 2014,195(3):435-452.
doi: 10.1007/s10681-013-1006-z
[6] Wang L X, Qiu J, Chang L F , et al. Assessment of wheat variety distinctness using SSR markers. Journal of Integrative Agriculture, 2015,14(10):1923-1935.
doi: 10.1016/S2095-3119(15)61057-7
[7] 王凤格, 田红丽, 赵久然 , 等. 中国328个玉米品种(组合)SSR标记遗传多样性分析. 中国农业科学, 2014,47(5):856-864.
doi: 10.3864/j.issn.0578-1752.2014.05.003
[8] 关荣霞, 刘燕, 刘章雄 , 等. 利用SSR方法鉴定大豆品种纯度. 分子植物育种, 2003(3):75-78.
doi: 10.3969/j.issn.1672-416X.2003.03.010
[9] 李俊芳, 张雪原, 孙世贤 , 等. 国家玉米主产区预试品种的SSR分析Ⅰ.预试品种的真实性和一致性评价. 玉米科学, 2006,14(6):38-42.
doi: 10.3969/j.issn.1005-0906.2006.06.010
[10] 王凤格, 赵久然, 郭景伦 , 等. 中国玉米新品种DNA指纹库建立系列研究Ⅰ.玉米品种纯度及真伪鉴定中SSR技术标准实验体系的建立. 玉米科学, 2003,11(1):3-6.
[11] 王凤格, 赵久然 . 玉米品种DNA 指纹鉴定技术-SSR标记的研究与应用.北京: 中国农业科学技术出版社, 2011.
[12] 赵久然, 孙世贤, 王凤格 . 中国玉米品种DNA指纹鉴定研究动态.北京: 中国农业科学技术出版社, 2008.
[13] 田大刚, 林艳, 刘华清 , 等. 123份水稻重要品种的SSR核心标记指纹分析. 分子植物育种, 2013(1):20-29.
[14] 庄杰云, 施勇烽, 应杰政 , 等. 中国主栽水稻品种微卫星标记数据库的初步构建. 中国水稻科学, 2006,20(5):460-468.
doi: 10.3321/j.issn:1001-7216.2006.05.002
[15] 陆光远, 伍晓明, 张冬晓 , 等. SSR标记分析国家油菜区试品种的特异性和一致性. 中国农业科学, 2008,41(1):32-42.
[16] 匡猛, 杨伟华, 许红霞 , 等. 中国棉花主栽品种DNA 指纹图谱构建及 SSR 标记遗传多样性分析. 中国农业科学, 2011,44(1):20-27.
doi: 10.3864/j.issn.0578-1752.2011.01.003
[17] 段艳凤, 刘杰, 卞春松 , 等. 中国88个马铃薯审定品种SSR 指纹图谱构建与遗传多样性分析. 作物学报, 2009,35(8):1451-1457.
doi: 10.3724/SP.J.1006.2009.01451
[18] 刘红艳, 左阳, 吴坤 , 等. 国家芝麻区域试验新品种(系)的DNA指纹分析. 作物学报, 2012,38(4):596-605.
doi: 10.3724/SP.J.1006.2012.00596
[19] 邱军 . 国家小麦品种区试审定现状和发展. 第十五次中国小麦栽培科学学生研讨会论文集. 2012.
[20] 季伟, 王立新, 孙辉 , 等. 小麦SSR分析体系的简化. 农业生物技术学报, 2007,15(5):907-908.
[21] Liu K, Muse S V . PowerMarker:an integrated analysis environment for genetic marker data. Bioinformatics, 2005,21(9):2128-2129.
doi: 10.1093/bioinformatics/bti282
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