Crops ›› 2017, Vol. 33 ›› Issue (3): 49-53.doi: 10.16035/j.issn.1001-7283.2017.03.010

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The Resistance to Soybean Cyst Nematode Race 1 of Transgenic Soybean with hrf2 Encoding HarpinXooc

Ren Guoyong,Li Wei,Zhang Lifeng,Wang Caijie,Dai Haiying,Wang Jinlong,Xu Ran,Zhang Yanwei   

  1. Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250131,Shandong,China
  • Received:2017-02-22 Revised:2017-04-06 Online:2017-06-15 Published:2018-08-26
  • Contact: Yanwei Zhang

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Abstract:

The Harpin protein could make plants produce broad-spectrum resistance to stress by activating the defense pathways of plants. HarpinXooc protein encoded by hrf2 gene has similar function to Harpin protein. In this study, the evaluation was performed to estimate the resistance to soybean cyst nematode (SCN) of transgenic soybean with hrf2 gene. The result showed that the SCN number of two lines of transgenic soybean with hrf2 gene was significantly lower than that of the receptor plant Tianlong 1, indicating that hrf2 gene might improve soybean resistance to SCN race 1.

Key words: Harpin, hrf2, Soybean, Soybean cyst nematode, Race 1

Table 1

Primer sequence"

引物名称Primer name 上游引物序列Forward primer sequence (5'-3') 下游引物序列Reverse primer sequence (5'-3') PCR产物PCR product (bp)
hrf2 CGGGATCCATGAACTCTTTGAACACACA CGAGCTCTTACTGCATTGATGCGCT 414
Bar CAGCTGCCAGAAACCCACGT CTGCACCATCGTCAACCACT 436

Table 2

The evaluation criterion of soybean resistance to SCN"

抗性评价Resistance evaluation 免疫Immunity 高抗Resistant 中抗Moderately resistant 感Susceptible 高感High susceptible
单株着生雌虫平均数
Average of cyst nematode per plant		 0
 0.1~3.0
 3.1~10.0
 10.1~30.0
 30.0以上

Fig.1

The PCR detection for hrf2 of transgenic soybean"

Fig.2

The PCR detection for Bar of transgenic soybean Positive: pCAMBIA3300::hrf2; M: D2000 of Tiangen; CK: Tianlong 1; A1-A6: Six plants of T2-24-1; B1-B5: Five plants of T2-5-1"

Fig.3

The detection of transgenic soybean by Bar strip CK: Tianlong 1; A1, A2, A4-A7: Six plants of T2-24-1; B2, B3, B6: Three plants of T2-5-1"

Fig.4

The resistance to PPT of transgenic soybean CK: Tianlong 1; A1, A2: Two plants of T2-24-1; B2, B3: Two plants of T2-5-1"

Table 3

The resistance to SCN of soybean plants"

株系Lines 观测数No. of plants 最大值Max 最小值Min 单株胞囊数No. of cytocyst per plant 抗性级别The resistance level
Peking 8 7 0 2.13±1.02 R
Lee 8 81 47 61.88±5.26 HS
Pickett 7 5 0 3.57±0.52 MR
PI88788 8 45 29 37.13±4.26 HS
PI90763 7 3 0 1.29±0.32 R
天隆一号Tianlong 1 5 47 35 43.00±2.10 HS
T2-24-1 32 47 5 21.66±13.10 S
T2-5-1 9 22 4 15.22±6.69 S

Fig.5

The resistance to SCN of transgenic soybean CK: Tianlong 1; **: Highly significant difference (t test, P≤0.01)"

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