Crops ›› 2016, Vol. 32 ›› Issue (2): 57-62.doi: 10.16035/j.issn.1001-7283.2016.02.010

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Study on Temporospatial Expression of PAL Gene Family in Soybean

Hou Peng,Liang Dong,Zhang Weiguo,Guo Changjun,Zhang Xiaoming,Cheng Zhang,Zhang Shuzhen,Li Dongmei,Li Wenbin,Zhang Dayong   

  1. Key Laboratory of Soybean Biology,Chinese Ministry of Education,Northeast Agricultural University,Harbin 150030,Heilongjiang,China
  • Received:2015-12-30 Revised:2016-03-14 Online:2016-04-15 Published:2018-08-26
  • Contact: Dayong Zhang

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Abstract:

Phenylalanine metabolic pathway is one of the most important secondary metabolic pathways in plant, and its final metabolites including isoflavones, phytoalexin, lignin and other secondary metabolites. Phenylalanine ammonia-lyase (PAL) is the initial enzyme and key enzyme in phenylalanine metabolic pathways. PAL gene family consists of eight members, In order to know their expression in soybean, this paper was to study the expressions of all the PAL family members by real-time quantitative PCR methods in soybean. Relative expression of PAL gene family in reproductive growth period is higher than that in vegetative growth period, and relative expression is higher in the leaf and grain. The relative expression of PAL1-1, PAL2-1 and PAL2-3 genes is significantly higher than other genes of PAL gene family in each organs of soybean. PAL1-1 gene expression of different periods is stable, and the express of PAL2-1 and PAL2-3 genes appeared successively to peak in the late reproductive growth. The above results show that the soybean PAL gene family members express in the different time or space. It has a certain time and space expression specificity.

Key words: Soybean, Phenylalanine ammonia-lyase, Gene families, Temporospatial expression

Table 1

Gene and q RT-PCR primers used in this study"

基因Gene 基因座位号Gene number 引物序列Primer sequences PCR产物长度PCR product size (bp)
PAL1-1 19G182300 F：TCCAATCTGTTGATTTTACTA
R：TATAATAGAACCAAAGCTCCG		 180
PAL1-2 03G181700 F：TCATTTGGTTTGTCTATCATTTAAC
R：CAAAGCTTCTGCAAACAACT		 101
PAL1-3 03G181600 F：GGGAAAATTATTGATCCTC
R：AATTAGGCTTTTCAAACTGA		 173
PAL2-1 10G058200 F：CATGGATAATACACGTTTG
R：CAGAACAATAAGATGCCA		 179
PAL2-2 20G180800 F：ACTGATAGTTACGGAGTCA
R：AGATTCTGTGCCATTACC		 129
PAL2-3 13G145000 F：CAATACTTGGCGAACCCG
R：TGGCAAAGTGCAATGAGG		 155
PAL2-4 10G209800 F：CCTCTACGTGGTACAATC
R：CAAAACTTCTGAAAGGAT		 276
PAL3 02G309300 F：AGTAATCTGCCGATGTTTGA
R：AGATAAGGCGTACACTGGAA		 129
Tua-5 F：TGCCACCATCAAGACTAAGAGG
R：ACCACCAGGAACAACAGAAGG		 113

Fig.1

The relative expression analysis of PAL gene families and its members in soybean"

Fig.2

The relative expression variation of PAL1-1，PAL2-1和PAL2-3 gene in soybean"

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