Crops ›› 2016, Vol. 32 ›› Issue (3): 21-26.doi: 10.16035/j.issn.1001-7283.2016.03.005

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Inheritance of Resistance to Race 4 of Cyst Nematode in Soybean

Zhang Haiping1,2,Wu Shufeng2,Wang Zhi2   

  1. 1Shanxi University,Taiyuan 030031,Shanxi,China
    2Institute of Crop Germplasm Resources,Shanxi Academy of Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,P.R.China/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement,Taiyuan 030031,Shanxi,China
  • Received:2016-04-13 Revised:2016-05-07 Online:2016-06-15 Published:2018-08-26

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Abstract:

Soybean cyst nematode (SCN) is a destructive pest in soybean production worldwide and causes great yield loss every year. There are 14 SCN races worldwide, among which 8 races are in china. Race 4 of SCN (SCN4), which is the predominant race in Huang-Huai-hai soybean production area, has the most virulence. A soybean cultivar-CBL, which was isolated from Chibuliuheidou with high resistance to SCN, but its growth period was shorter than Chibuliuheidou. In this paper, genetic model of CBL was studied, which will help for cloning of resistant gene and resistant marker exploitation. Three generations (F1, F2, F2:3 ) were generated from the crosses between CBL and pin 75-14, which was a high susceptible to SCN4. Genetic model was analyzed by the method of mixed major gene plus polygene genetic model. The resistant inheritance to SCN4 was controlled by two additive-dominance-espistatic major genes and additive-dominance-espistatic polygenes. The F2 and F2:3 heritabilities of major genes were estimated to be 64.47% and 75.99%, respectively. So resistance plants can be selected in early filial generation.

Key words: Soybean, Soybean cyst nematode, Major gene plus polygene, Inheritance

Table 1

The SCN4 disease index of each generation"

世代
Generation		 病情指数分布Disease level distribution(株) 总数
(株)
Total		 平均病情指数(%)
Disease index		 标准差
SD
0~
10%		 10.1%~
20%		 20.1%~
30%		 30.1%~
40%		 40.1%~
50%		 50.1%~
60%		 60.1%~
70%		 70.1%~
80%		 80.1%~
90%		 90.1%~
100%		 >100%
P1 10 10 192.85 8.14
P2 10 10 0.28 0.13
F1 1 9 10 117.66 16.76
F2 10 6 4 9 10 5 6 6 6 4 52 118 102.00 55.77
F2∶3 8 6 3 4 6 17 13 13 6 11 31 118 72.03 27.25

Table 2

Maximum likelihood values and AIC value of genentic models from resistance"

模型代码
Model code		 模型
Model		 极大似然值
Max likelihood value		 AIC值
AIC value
A1 1MG-AD -4 039.66 8 087.326
A2 1MG-A -4 041.22 8 088.441
A3 1MG-EAD -4 040.94 8 087.885
A4 1MG-AEND -4 040.94 8 087.881
B1 2MG-ADI -3 785.66 7 591.325
B2 2MG-AD -3 890.82 7 793.635
B3 2MG-A -3 842.68 7 693.352
B4 2MG-EA -4 281.48 8 568.954
B5 2MG-AED -3 918.90 7 845.809
B6 2MG-EEAD -4 525.45 9 056.901
C0 PG-ADI -3 855.96 7 731.914
C1 PG-AD -3 924.15 7 862.299
D0 MX1-AD-ADI -3 814.41 7 648.811
D1 MX1-AD-AD -3 911.74 7 839.481
D2 MX1-A-AD -3 924.04 7 862.086
D3 MX1-EAD-AD -3 922.40 7 858.794
D4 MX1-AEND-AD -3 925.51 7 865.020
E0 MX2-ADI-ADI -3 777.41 7 586.823
E1 MX2-ADI-AD -3 779.71 7 587.411
E2 MX2-AD-AD -3 911.74 7 843.481
E3 MX2-A-AD -3 896.45 7 808.902
E4 MX2-EAED-AD -3 924.00 7 861.996
E5 MX2-AED-AD -3 880.57 7 777.141

Table 3

Fitness test results of models B1, E0 and E1"

模型Model 世代Generation U12 U22 U32 nW2 Dn
B1 P1 5.3455(0.0208) 21.3231(0.0000) 90.5607(0.0000) 8.4035(0.0121) 0.0096(1.0000)
P2 5.5307(0.0187) 21.6204(0.0000) 90.0765(0.0000) 8.4195(0.0122) 0.0096(1.0000)
F1 6.4420(0.0111) 24.9129(0.0000) 102.7199(0.0000) 9.4931(0.0166) 0.0086(1.0000)
F2 0.3390(0.5604) 1.3029(0.2537) 5.3397(0.0208) 3.1920(0.0002) 0.0042(1.0000)
F2∶3 1.1842(0.2765) 0.0000(0.9974) 17.8719(0.0000) 3.2920(0.0002) 0.0584(0.3824)
E0 P1 3.5646(0.0590) 18.2294(0.0000) 95.3780(0.1035) 8.2496(0.0115) 0.0096(1.0000)
P2 3.5646(0.0590) 18.2294(0.0874) 95.3780(0.5347) 8.2496(0.1215) 0.0096(1.0000)
F1 2.9674(0.0850) 18.6210(0.0000) 112.1310(0.0000) 9.1941(0.0153) 0.0086(1.0000)
F2 1.9975(0.1576) 2.2509(0.1335) 0.2782(0.5979) 3.5021(0.3253) 0.0042(1.0000)
F2∶3 0.0740(0.7855) 0.6536(0.4188) 18.3838(0.0000) 3.1149(0.0902) 0.0397(0.8372)
E1 P1 4.1602(0.0414) 19.3183(0.0000) 93.7310(0.0000) 8.3011(0.0117) 0.0096(1.0000)
P2 4.1602(0.0414) 19.3183(0.0000) 93.7310(0.0000) 8.3011(0.0117) 0.0096(1.0000)
F1 7.6119(0.0058) 26.7112(0.0000) 99.7542(0.0000) 9.5936(0.0170) 0.0086(1.0000)
F2 0.0866(0.7685) 0.3651(0.5457) 12.6517(0.0004) 3.1534(0.0002) 0.0042(1.0000)
F2∶3 1.2685(0.2600) 3.2737(0.0704) 8.2673(0.0040) 3.2366(0.0002) 0.0603(0.3435)

Table 4

Estimate of genentic parameter of fitted model"

一阶遗传参数
1st order genentic
parameter		 估计值
Estimate		 二阶遗传参数
2st order genentic
parameter		 估计值Estimate
F2 F2∶3
m1(m) -50.12 σf2 2 255.52 518.02
m2 9.79 σmg2 4 092.64 1 640.26
m3 18.12 σpg2 1 737.52 0
m4 78.99 σp2 6 348.18 2 158.28
m5 22.92 σ2 518.02 518.02
da(d) 18.11 hmg2 64.47% 75.99%
db 18.43 hpg2 27.37% 0
ha(h) -14.51
hb 54.48
i 18.09
jab 55.18
jba -15.66
l -58.73
[1] 王敬强, 程大新, 宛煜嵩 , 等. 应县小黑豆对大豆孢囊线虫4号生理小种抗性的遗传分析. 中国农学通报, 2001,17(6):12-15.
[2] 刘维志, 段玉玺 .植物病原线虫学.北京:中国农业出版社, 2000.
[3] 孙漫红, 刘杏忠, 缪作清 . 大豆胞囊线虫病生物防治研究进展. 中国生物防治, 2000,16(3):136-141.
doi: 10.3321/j.issn:1005-9261.2000.03.010
[4] Konanani B , Liphadzi, Al-Khatib, et al. Soil microbial and nematode communities as affected by glyphosate and tillage practices in a glyphosate -resistant cropping system. Weed Science, 2005,53:536-545.
doi: 10.1614/WS-04-129R1
[5] 许艳丽, 王丽芳, 战丽莉 .大豆胞囊线虫病研究进展(续一).大豆科技,2010(1):21-24.
[6] 卢为国, 盖钧镒, 李卫东 . 黄淮地区大豆胞囊线虫生理小种的抽样调查与研究. 中国农业科学, 2006,39(2):306-312.
doi: 10.3321/j.issn:0578-1752.2006.02.013
[7] Caldwell B E, Brim C A . Inheritance resistance of soybean to the Heterodera glycines. Agronomy Journal, 1960,52:633-636.
[8] 王树峰, 李卫东, 刘世涛 .应县小黑豆对大豆胞囊线虫( SCN)1号生理小种的抗性遗传分析.河南农业科学,2004(5):28-31.
doi: 10.3969/j.issn.1004-3268.2004.05.008
[9] Yue P, Sleper D A ,Rao-Arelli A P.Genetic analysis of resistance to soybean cyst nematode in PI438489B. Euphytica, 2000,116(2):181-186.
doi: 10.1023/A:1004054731652
[10] Hartwig E E, Epps J M . An additional gene for resistance to soybean cyst nematode Heterodera glycines. Phytopathology, 1970,60:584.
[11] Rao-Arelli A P, Anand S C, Wrather J A . Soybean resistance to soybean cyst nematode race 3 is conditioned by an additional dominant gene. Crop Science, 1992,32(4):862-864.
doi: 10.2135/cropsci1992.0011183X003200040005x
[12] Rao-Arelli A P . Inheritance of resistance to Heterodera glycines race 3 in soybean accessions. Plant Diseases, 1994,78(9):898-900.
doi: 10.1094/PD-78-0898
[13] Lu P, Shannon J G . Genetics of cyst nematode resistance in soybean PI467312 and PI507354. Euphytica, 2006,149(3):259-265.
doi: 10.1007/s10681-005-9073-4
[14] 刘维志, 洪权春, 刘哗 , 等. 中国小黑豆对大豆胞囊线虫3号生理小种的抗性遗传研究. 沈阳农业大学学报, 1996,27(1):31-34.
[15] 李莹, 李源萍, 张听艳 , 等. 大豆品种对孢囊线虫4号生理小种抗性的遗传研究. 大豆科学, 1996,15(3):191-196.
[16] 卢为国, 盖钧镒, 李卫东 . 大豆对胞囊线虫1号和4号生理小种抗性的遗传分析. 作物学报, 2006,32(5):650-655.
[17] Myers G O, Anand S C . Inheritance of resistance and genetic relationships among soybean plant introductions to race of soybean cyst nematode. Euphytica, 1991,55(3):197-201.
doi: 10.1007/BF00021239
[18] Vuong T D, Sleper D A, Shannon J G , et al. Novel quantitative trait loci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe ) in soybean PI 567516C. Theoretical and Applied Genetics, 2010,121(7):1253-66.
doi: 10.1007/s00122-010-1385-7
[19] Elston R C, Steward J . The analysis of quantitative trait for simple genetic models from parental,F1 and backcross data. Genetics, 1973,73(4):695-711.
[20] Elston R C . The genetic analysis of quantitative trait difference between two homozygouse lines. Genetics, 1984,108(3):733-744.
pmid: 6500262
[21] Knott S A, Haley C S, Thompson R . Methods of segregation analysis for animal breeding data,parameter estimates. Heredity, 1992,68(4):313-320.
doi: 10.1038/hdy.1992.45 pmid: 1563967
[22] Shoukri M M, Mclachlan G J . Paramtirc estimation in a mixture model with application to nuclear family data. Biometrics, 1994,50(1):128-139.
doi: 10.2307/2533203 pmid: 8086597
[23] 王建康, 盖钧镒 . 利用杂种F2世代鉴定数量性状主基因-多基因混合遗传模型并估计其遗传效应. 遗传学报, 1997,24(5):432-440.
[24] 王建康, 盖钧镒 . 数量性状主-多基因混合遗传的P1、P2、F1、F2和F2∶3联合分析方法. 作物学报, 1998,24(6):651-659.
[25] 章元明, 盖钧镒, 张孟臣 . 利用P1、P2、F1、F2和F2∶3世代联合分离分析. 西南农业大学学报, 2000,22(1):6-9.
[26] 章元明, 盖钧镒 . 利用P1、P2、F1、F2和F2∶3家系五世代联合分离分析的拓展. 生物数学学报, 2002,17(3):363-368.
doi: 10.3969/j.issn.1001-9626.2002.03.018
[27] 盖钧镒, 章元明, 王健康 .植物数量性状遗传体系.北京:科学出版社, 2003.
[28] 卢为国, 袁道华, 李金英 , 等.大豆抗胞囊线虫基因不同世代遗传率的变化.河南农业科学,2010(2):24-27.
doi: 10.3969/j.issn.1004-3268.2010.02.006
[29] 张翠翠, 代帅帅, 张兴伟 , 等. 烤烟CMV抗性主基因+多基因混合遗传模型分析. 植物遗传资源学报, 2014,15(6):1278-1286.
doi: 10.13430/j.cnki.jpgr.2014.06.016
[30] 张允楠, 曹齐卫, 李立斌 , 等. 黄瓜叶面积的主+多基因混合遗传模型分析. 园艺学, 2015,42(5):897-906.
[31] 吴书峰, 王志, 乔治军 , 等. 大豆抗胞囊线虫4号生理小种转录组测序及分析. 农业生物技术学报, 2016,24(4):519-529.
doi: 10.3969/j.issn.1674-7968.2016.04.006
[32] Riggs R D, Schmitt D P . Complete characterization of the race scheme for Heterodera glycines. Joural of Nematology, 1988,20(3):392-395.
doi: 10.1016/0022-2011(88)90126-7 pmid: 19290228
[33] 王志, 张海平, 李原萍 .一种在大豆抗胞囊线虫研究中抗病植株移植方法.中国,专利号:201310592034.X.
[34] 刘兵 . 植物数量性状分离分析Windows软件包SEA的研制. 南京:南京农业大学, 2013.
doi: 10.7685/j.issn.1000-2030.2013.06.001
[35] 赵桂云, 王继安, 李文滨 , 等. 大豆抗食心虫主基因+多基因混合遗传模型的五世代联合分析. 大豆科学, 2014,33(8):301-304.
doi: 10.11861/j.issn.1000-9841.2014.03.0301
[36] Dias W P ,CamposV P,Kiihl R A S,et al.Genetic controln soybean of resistance to soybean cyst nematode race4+. Euphytica, 2005,145(3):321-329.
doi: 10.1007/s10681-005-1944-1
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