Crops ›› 2020, Vol. 36 ›› Issue (1): 22-28.doi: 10.16035/j.issn.1001-7283.2020.01.005

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Expression Analysis and Interaction Prediction of Maize Glucose Transporter Gene ZmGLUT-1

Zhang Xin1,Cao Liru1,2,Wei Liangming1,Zhang Qianjin1,Zhou Ke1,Wang Zhenhua1(),Lu Xiaomin1()   

  1. 1Grain Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, Henan, China
    2Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2019-07-10 Revised:2019-09-29 Online:2020-02-15 Published:2020-02-23
  • Contact: Zhenhua Wang,Xiaomin Lu E-mail:wzh201@126.com;luxiaomin2004@163.com

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Abstract:

Glucose transporter (GLUT1) maintains cell stability by maintaining glucose concentration on both sides of the cell membrane, which plays an important role in plant stress tolerance. In this study, GLUT1 gene (1 263bp) was cloned from the ‘zheng 36’, which was temporarily named ZmGLUT-1 gene. The promoter cis element analysis revealed that the gene contains various binding sites in response to stress, hormone signaling, and photo-stimulation response. InSilico analysis indicates that the protein is a hydrophobic protein with 12 transmembrane domains, mainly in the alpha helix structure, and subcellular localization on the plasma membrane. The result of qRT-PCR indicated that the gene was a constitutively expressed gene and was highly expressed in leaf tips and embryos. It was also found that the gene was down-regulated by abscisic acid (ABA) and PEG treatment, the expression after rehydration increased but could not reach normal level. Mutual protein analysis revealed that perhaps ZmGLUT-1 protein and the interaction proteins constitute a regulatory network. The above studies indicate that ZmGLUT-1 gene is associated with drought stress. These results provide information for further exploration of GLUT1 function and mechanism in plants, and for screening drought-resistant high-quality breeding germplasms.

Key words: Maize, Glucose transporter, Drought and ABA stress, Cell stability

Fig.1

The cDNA electrophoresis of ZmGLUT-1 gene M is Trans5K DNA marker, and lane 1 is the PCR amplification product of ZmGLUT-1 gene"

Fig.2

The phylogenetic tree and conserved functional motifs of the ZmGLUT-1 protein and other species proteins Pahal.D02166.2 stands for Juglans regia Linn; Pavir.lb01737.1 stands for Panicum virgatum L.; Seita.9G395700.2 stands for Setaria italica; Sobic.001G362500.1 stands for Sorghum bicolor (L.); LOC_Os03g24860.1 stands for Oryza sativa Linn.; Bradi1g61757.2 stands for Brachypodium distachyon (L.); AT2G48020.2 stands for Arabidopsis thaliana (Linn.); Gorai.005G056400.1 stands for Gossypium hirsutum Linn.; Glyma.13G213200.4 stands for Glycine max (Linn.)"

Table 1

The cis-element analysis of ZmGLUT-1 gene promoter"

元件Element 来源植物Source plant 位点Site 序列Sequence 功能Function
ABRE Oryza sativa 1 525 GCCGCGTGGC cis-acting element involved in the abscisic acid responsiveness
AuxRR-core Nicotiana tabacum 1 011 GGTCCAT cis-acting regulatory element involved in auxin responsiveness
CAT-box Arabidopsis thaliana 1 640 GCCACT cis-acting regulatory element related to meristem expression
CGTCA-motif Hordeum vulgare 494 CGTCA cis-acting regulatory element involved in the MeJA-responsiveness
GATA-motif Arabidopsis thaliana 1 106 GATAGGA part of a light responsive element
GT1-motif Arabidopsis thaliana 24 GGTTAA light responsive element
I-box Zea mays 1 285 GGATAAGGTG part of a light responsive element
O2-site Zea mays 1 286 GATGATGTGG cis-acting regulatory element involved in zein metabolism regulation
Sp1 Oryza sativa 1 459 GGGCGG light responsive element
TGA-element Brassica oleracea 428 AACGAC auxin-responsive element
TGACG-motif Hordeum vulgare 494 TGACG cis-acting regulatory element involved in the MeJA-responsiveness

Fig.3

Expression pattern of ZmGLUT-1 gene in different organs of maize"

Fig.4

Expression pattern of ZmGLUT-1 gene in response to PEG and ABA"

Fig.5

Subcellular localization of ZmGLUT-1 protein in tobacco"

Table 2

Functional interaction protein prediction of ZmGLUT-1"

基因号
Gene ID		 基因描述
Gene description		 功能结构域
Function domain		 氨基酸
Amino acid		 互作系数
Interaction coefficient
GRMZM2G138423 ADP, ATP carrier protein ADP, ATP transporter on adenylate translocase 328 0.82
GRMZM2G100976 PhotosystemI1 NifU-like domain 266 0.80
GRMZM5G825759 TP synthase B chain ATP synthase B/B′ CF(0) 216 0.80
GRMZM2G073244 UDP-glycosyltransferase 84A1 Glycosyltransferase_GTB_type 481 0.80
GRMZM2G124321 UvrB/uvrC motif-containing protein UvrB/uvrC motif 334 0.72
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