Crops ›› 2019, Vol. 35 ›› Issue (4): 183-190.doi: 10.16035/j.issn.1001-7283.2019.04.028

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Response of Cutin Synthetic Genes of Foxtail Millet to Drought Stress

Yue Linqi,Shi Weiping,Guo Jiahui,Guo Pingyi,Guo Jie   

  1. College of Agronomy, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2019-03-25 Revised:2019-06-13 Online:2019-08-15 Published:2019-08-06
  • Contact: Pingyi Guo,Jie Guo

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Abstract:

Drought is an important influence factor on the yield of foxtail millet. The drought-resistant foxtail millet variety Chigu 16 (M79) and its female parent Chigu 10 (E1) and male parent Chenggu 8 (H1) were used as test materials in this study. Natural drought treatment at the seedling stage was adopted and a control was set up. Leaves were taken off on the 6th day of drought treatment (soil moisture content was about 20%) for transcriptome sequencing. The physiochemical properties of genes were analyzed on ExPASY, and gene structures were detected on GSDS 2.0. A phylogenetic tree was constructed on MAGA 7.0, the transmembrane domain was predicted on TMHMM Server v. 2.0 and the secondary structure of proteins was predicted using SOPMA. It was found that the proteins encoded by the 8 cutin synthetic genes of foxtail millets were all hydrophilic, and the 8 genes were all acidic. After the drought stress treatment, the expressions of the 8 genes changed in different trends among M79, E1 and H1, and Seita.8G128800 and Seita.7G197000 were significantly upregulated. Four of the eight genes were homologous with corn genes, three of the eight genes were homologous with rice genes, and a part of coding proteins had a transmembrane domain. The cutin synthetic genes play a role in the drought response of foxtail millet, but their regulation mechanism is complex. Our findings provide theoretically underlie for further exploration into the relationship between foxtail millet cutin and drought resistance.

Key words: Foxtail millet, Cutin, Drought resistance, Bioinformatics analysis

Fig.1

Structure of cutin synthetic genes in foxtail millet"

Fig.2

Analysis of hydrophilicity and hydrophobicity of proteins encoded by cutin synthetic genes in forctail millet"

Table 1

Basic information of cutin synthetic genes in foxtail millet"

基因名称
Gene name		 基因长度(bp)
Length of gene		 CDS长度(bp)
Length of CDS		 染色体
Chromosome		 氨基酸数目
Number of amino acids		 等电点
PI		 分子量(Da)
Molecular weight
Seita.1G219200 2 276 1 590 1 529 4.83 187 041.49
Seita.8G128800 5 998 1 365 8 454 4.64 499 484.30
Seita.7G197000 1 629 1 629 7 542 4.86 134 851.34
Seita.1G237600 6 547 1 863 1 620 4.67 545 565.01
Seita.2G306800 3 599 1 509 2 502 4.83 299 516.29
Seita.6G138200 4 194 1 755 6 584 4.76 348 222.58
Seita.1G362700 3 031 1 881 1 626 4.75 253 210.31
Seita.6G244000 4 219 1 494 6 497 4.76 351 628.77

Fig.3

Cluster heatmap of differential expressions of cutin synthetic genes in M79, H1 and E1 after drought treatment"

Fig.4

The evolution tree of cutin synthetic genes in foxtail millet"

Fig.5

Transmembrane domain prediction of cutin synthetic genes in foxtail millet"

Table 2

Prediction of secondary structure of foxtail millet cutin synthetic gene protein %"

蛋白名称
Protein name		 α螺旋
Alpha helix		 β转角
Beta turn		 无规则卷曲
Random coil		 延伸链
Extended strand
Seita.1G219200 51.04 9.26 13.80 25.90
Seita.8G128800 40.62 9.27 31.35 18.76
Seita.7G197000 45.57 11.99 26.01 16.42
Seita.2G306800 50.60 7.97 24.90 16.53
Seita.6G138200 27.05 12.16 35.45 25.34
Seita.1G362700 45.05 7.19 29.39 18.37
Seita.6G244000 43.86 7.65 28.37 20.12
Seita.1G237600 42.26 8.71 28.23 20.81
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