Crops ›› 2021, Vol. 37 ›› Issue (1): 150-159.doi: 10.16035/j.issn.1001-7283.2021.01.021

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Effects of Nitrogen and Nitrogen Forms on Photosynthetic Fluorescence Parameters of Chlorophyll in Flue-Cured Tobacco Leaves

Huang Dan1(), Yang Fuwen1, Liu Lin2, Wang Yue1, He Guoqiang2, Wang Hongrui1, Sun Guangyu1, Ao Hong1()   

  1. 1College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China
    2Mudanjiang Institute of Tobacco Sciences, Harbin 150076, Heilongjiang, China
  • Received:2020-07-13 Revised:2020-10-28 Online:2021-02-15 Published:2021-02-23
  • Contact: Ao Hong E-mail:2018120877@nefu.edu.cn;aohong2003@163.com

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Abstract:

To explore the effects of nitrogen application and nitrogen forms on the photosynthetic capacity of flue-cured tobacco (Nicotiana tabacum L.) seedlings and to provide a scientific basis for rational application of nitrogen fertilizers to flue-cured tobacco, the two varieties of tobacco Longjiang911 and Longjiang0520 in the absence of N (CK), nitrate nitrogen (NO3--N), ammonium nitrogen (NH4+-N), and ammonium nitrate mixed nitrogen (NO3--N:NH4+-N=1:1) processing the photosynthetic gas exchange parameters and the changing rule of the fluorescence kinetics curve was studied. The results showed that the net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) of the nitrogen treatments were higher than those of CK, and with the extension of the cultivation time, the increase range was increased, and the difference was reached up to a significant level (P < 0.05). Nitrogen application generally increased Fv/Fm and PIabs of the two flue-cured tobacco varieties, indicating that nitrogen promoted the activity of PSⅡ reaction centers in flue-cured tobacco seedling leaves; at the same time, nitrogen application reduced VJ and VI. The comparison of fluorescence parameters under three different nitrogen forms showed that the fluorescence parameters Fv/Fm and PIabs of flue-cured tobacco leaves treated by NH4+-N were relatively lower, while VJ and VI were relatively higher, indicating that NH4+-N was relatively weak in improving the photosynthetic performance of PSⅡ. By combining both photosynthetic gas exchange parameters and chlorophyll fluorescence parameters, it could be seen that photosynthetic performances of Longjiang911 treated with NO3--N and Longjiang0520 treated with NO3--N:NH4+-N (1:1) were better.

Key words: Nitrogen form, Flue-cured tobacco, Rapid fluorescence kinetics curve

Table 1

Principal component ratio and number of moles of nitrate nitrogen and ammonium nitrogen nutrient solution under different treatments mmol/L"

营养液主要成分
Nutrient solution
principal component		 NO3--N∶NH4+-N
0∶0
(CK)		 100∶0
(T1)		 0∶100
(T2)		 50∶50
(T3)
Ca(NO3)2 0.000 0.500 0.000 0.375
KNO3 0.000 0.500 0.000 0.000
(NH4)2SO4 0.000 0.000 0.750 0.375
K2SO4 0.250 0.000 0.250 0.250
CaSO4 1.000 0.000 1.000 0.125
MgSO4 1.000 1.000 1.000 1.000

Table 2

Effects of different nitrogen forms on photosynthetic gas parameters of leaves of two flue-cured tobacco varieties"

品种
Variety		 处理
Treatment		 Pn [μmol/(m2·s)] Tr [mmol/(m2·s)] Gs [mol/(m2·s)] Ci (μmol/mol)
处理后15d 处理后30d 处理后15d 处理后30d 处理后15d 处理后30d 处理后15d 处理后30d
LJ911 CK 5.19±0.33d 2.04±0.22d 2.99±0.24d 0.66±0.04c 0.10±0.01d 0.02±0.00d 348.87±5.93abc 313.78±35.04b
T1 9.61±0.97b 12.15±0.38a 5.48±0.34a 3.58±0.35a 0.31±0.03a 0.33±0.03a 379.20±8.37a 333.78±14.97ab
T2 5.09±0.28d 5.49±0.56c 0.92±0.10ef 3.03±0.33b 0.06±0.01e 0.24±0.03c 336.07±19.64bc 362.33±21.02a
T3 7.35±0.74c 8.65±0.84b 3.91±0.40c 3.68±0.33a 0.12±0.01c 0.27±0.03bc 361.00±39.38ab 339.17±16.54ab
LJ0520 CK 1.84±0.16e 0.45±0.04e 0.58±0.05f 0.18±0.02d 0.02±0.00f 0.01±0.00d 333.27±12.68bc 355.11±27.01a
T1 2.48±0.26e 9.08±0.90b 1.10±0.13e 3.50±0.37a 0.03±0.00ef 0.27±0.03bc 316.60±11.38c 354.49±12.37a
T2 5.83±0.31d 2.63±0.11d 1.31±0.13e 0.69±0.02c 0.06±0.01e 0.03±0.00d 383.20±39.33a 273.36±8.55c
T3 11.36±1.08a 11.82±1.10a 4.45±0.43b 3.85±0.29a 0.24±0.10b 0.28±0.02b 337.47±9.36bc 312.62±12.25b

Fig.1

Effects of different nitrogen forms on OJIP curves of leaves of two flue-cured tobacco varieties Different lowercase letters indicate significant differences between different treatments of the same measurement (P < 0.05)"

Fig.2

Effects of different nitrogen forms on the relative variable fluorescence intensity of standardized J and I points in two flue-cured tobacco leaves"

Fig.3

The influence of different nitrogen forms on PSⅡ photochemical activities of leaves of two flue-cured tobacco varieties"

Fig.4

Effects of different nitrogen forms on the relative variable fluorescence intensity at point K and point L of leaves of two flue-cured tobacco"

Table 3

Correlation analysis result of gas exchange parameters and chlorophyll fluorescence parameters"

参数Parameter Pn Gs Ci Tr Fv/Fm PIabs Fo Fm
Pn -1
Gs -0.899** -1
Ci -0.466 -0.447 -1
Tr -0.871** -0.943** -0.456 -1
Fv/Fm -0.499 -0.559 -0.863** -0.506 -1
PIabs -0.690 -0.684 -0.766* -0.711* -0.799* 1
Fo -0.452 -0.520 -0.137 -0.422 -0.114 0.346 1
Fm -0.616 -0.664 -0.126 -0.574 -0.155 0.592 0.959** 1
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